Triticale is an important cereal crop grown throughout the world. Research showed that the regen-eration of young plants from mature embryos triticale depended on genotypic characteristics. The fre-quency of callusogenesis varied depending on the genotype and was: 188 TR5027 - 80.19%, Ingen 93 standard - 92.02% and Ingen 35 - 98.45%, and the frequency of rhizogenesis compared to embryogenesis proved to be high and constituted on average 57.35%. Only in 34.53% of the morphogenic callus, the de-velopment was of the embryoid type. The average frequency of regeneration was 35.07%. The dispersive analysis of the obtained results shows a significant influence of the genotype in establishing a positive callusogenetic response (P <0.001), the influence power being 76.04%.