In vitro cultivation and micropropagation of endangered valuable species of medicinal plants is one of the alternative methods of modern biotechnology. This method allows obtaining a large number of identical plants in a short time, contributing to the conservation of natural populations. Among them is Rhodiola rosea L. (golden root), which is considered an adaptogenic plant with biological activities, which include anti-aging, anti-inflammation, anti-stresses, antioxidant, etc. This paper presents the process of micropropagation of R. rosea plants by activating axillary buds. To initiate the in vitro culture, mature seeds collected from the populations of the Carpathian Mountains, Romania were used. R. rosea seeds were cultured in Murashige and Skoog (MS) medium suplimented with different concentration of growth regulators (cytokinins and auxins) either singly or in combination to find out a suitable medium for shoot multiplication. Prolific multiplication of shoots was performed on medium with increasing Ca (2+) concentration and fortified with benzyladenine (BA) and indole-3-acetic acid (IAA). The optimal combination of these plant growth regulators to induce the highest number of shoots occurred when a ratio of 2 parts BA to IAA 1 part was maintained. As a result of micropropagation, we managed to obtain proliferation rates of tens of shoots per explant. Data for percentage shoot regeneration, shoot number per explant and shoot length was recorded after 6 weeks of culture. For complete plant development regenerated shoots were excised and transferred to rooting medium comprising of ½ MS. Root growth in terms of number and length were recorded from after 4 weeks of culturing. The obtained results suggest that the micropropagation method is an applicable tool, not only for the reproduction of R. rosea, but also for the conservation of endangered species from this group of plants.