It is known that actinobacteria, being in the rhizosphere and synthesize various physiologically active substances, play an essential role in plant life. Like all other soil elements, actinobacteria enter into relationships with surrounding organisms, including plants. In the literature there is a lot of data on the positive effect of pure cultures of bacteria, fungi and actinobacteria on the growth and yield of plants. Microbes – activators increase the percentage of seed germination, accelerate the growth of seedlings, and often change the nature of biochemical processes. As a rule, microorganism culture supernatants are beneficial only in small doses. The object of research was actinobacteria of the genus Streptomyces, isolated by us – Streptomyces gougerotii CNMN-Ac-14 and its natural variants. Streptomycete strain and their natural variants were grown in a liquid complex medium M-I (the main source of C and N is corn flour) for 5 days in flasks on a vibrating table to obtain a complex of exometabolites (EM) containing growth-regulating substances. Triticale seeds were soaked in aqueous solutions of EM at a dilution of 1 : 200. A series of experiments was carried out to determine the change in the main physiological parameters of triticale seedlings soaked in solutions of the studied streptomycete strain and its natural variants. It was found that after the treatment of triticale seeds with solutions of EM of the studied strains and its variants, the increase in the weight of raw roots occurred under the influence of EM of the natural variant 16 of S. gougerotii CNMN-Ac-14. An increase in the weight of raw shoots was observed after treatment of seeds with solutions of S. gougerotii CNMN-Ac-14 and its variant 16: under the influence of the EM the main strain (by 28.57% compared to the control), and EM variant 16 – only by 14.28% compared to the control. The average number of roots increased after the treatment of triticale seeds with solutions of EM strain S. gougerotii CNMN-Ac-14 (by 14.94%). The average length of the main root increased to a greater extent under the influence of EM variant 16 (by 23.38%), while under the influence of EM of the main strain, the effect was less (by 13.5%). Thus, the experiments showed that the treatment of triticale seeds with solutions of streptomycetes EM isolated from the soil of Moldova, and their natural variants contributed mainly to the development of the root system.