Storage potential of flax seeds - indicator of genotype viability under ex situ conservation
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582.751.4:581.48:57.085.2 (1)
Botanică sistematică (850)
Morfologia plantelor. Anatomia plantelor (111)
Tehnici biologice, echipament şi metode experimentale în general (194)
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KORLATEANU, L., KUTSITARU, D., GANEA, Anatolie. Storage potential of flax seeds - indicator of genotype viability under ex situ conservation. In: International Congress of Geneticists and Breeders from the Republic of Moldova, Ed. 11, 15-16 iunie 2021, Chişinău. Chișinău, Republica Moldova: Centrul Editorial-Poligrafic al Universităţii de Stat din Moldova, 2021, Ediția 11, p. 84. ISBN 978-9975-933-56-8. DOI: https://doi.org/10.53040/cga11.2021.062
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International Congress of Geneticists and Breeders from the Republic of Moldova
Ediția 11, 2021
Congresul "International Congress of Geneticists and Breeders from the Republic of Moldova"
11, Chişinău, Moldova, 15-16 iunie 2021

Storage potential of flax seeds - indicator of genotype viability under ex situ conservation

DOI: https://doi.org/10.53040/cga11.2021.062
CZU: 582.751.4:581.48:57.085.2

Pag. 84-84

Korlateanu L., Kutsitaru D., Ganea Anatolie
 
Institute of Genetics, Physiology and Plant Protection
 
Disponibil în IBN: 16 iunie 2021


Rezumat

The purpose of this research was to study after accelerated aging (AA)-test of flax seeds the morphophysiological and biochemical parameters, which allow characterization of storage potential (SP) of collective accessions in active collections of the gene bank for ex situ conservation, and to reveal genotypic differences of genotypes by this trait. Flax genotypes were grouped by their potential capability to preserve viability of samples exposed to high temperature and humidity. Objects of studies were collection accessions of flax (fam. Linaceae, genus Linum, species Linum usitatissimum L.) from active collection of the plant gene bank. SP of seeds was determined by two tests: test for accelerated aging (AA) of seeds by Hampton and TeKrony’s method and test for electrical conductivity of solutions (EC). AA-test of seeds was conducted under the increased air temperature (42-43оC), air humidity (90-100%), aging time was 72 hours. After this test, various morphophysiological parameters of seeds and seedlings were measured according to the International Rules of ISTA: germinating power and germinability of seeds, radicle length, fresh and dry biomass of radicles. With regard to biochemical parameters, the activity of the peroxidase enzyme (PO) was measured in radicles of seedlings. Aged flax seeds were sprouted in Petri dishes in thermostat at 25оC. Experimental data were processed using the software Statistica 7. According to the set of different parameters of flax seeds and seedlings after the accelerated aging of seeds, the following flax genotypes had the highest storage potential: Duflavin №5, Pskovskiy L 3-2, B 36, Нoncheberger, and the lowest storage potential was found in MDI 05608 and MDI 05609 samples. Use of accelerated aging test of seeds and determination of their morphophysiological and biochemical parameters allowed assessment of storage potential of flax collection accessions from the active collection of the gene bank. After conduction of test for accelerated aging of seeds there was revealed a significant positive correlation of germinability of flax seeds with germinating power, radicle length, fresh and dry biomass of seedlings.