Heart rate (HR) is an ECG variable that reflects of the function of sinus node automatism and the conduction system. Higher heart rate associated with increased risk of cardiovascular morbidity and mortality. Genome-wide association studies have identified association of a nonsynonymous variant rs365990 [G] in MYH6 and increased heart rate in individuals of European ancestry. The aim of the study was to determine the distribution of rs365990 variants that modulate heart rate in young population of the Republic of Moldova. Study population was construct of 700 students from Nicolae Testemitsanu State University of Medicine and Pharmacy, aged between 19-25 years. Twelve-lead ECGs were obtained in resting conditions. Genotyping of rs365990 in MYH6 gene was performed using QuantStudio 6 Flex instrument to detect for distribution of the genetic variants by TaqMan SNP Genotyping Assay. The rs365990 genotypes A/A, A/G, G/G distributions' among a participants was 41.8%, 45%, 13.2% respectively (χ2 = 0.260, p = 0.609). The allele frequencies for A and G were 64% and 36% respectively. The frequencies of the A/G and G/G genotypes were higher in participants with HR >100 bpm than in participants with normal HR (60 – 100 bpm) (p = 0.05). The minor allele frequency (MAF) higher in participants with HR >100 bpm (0.50) than in participants with normal HR (0.3555). The minor allele frequency (MAF) in young Moldavian population was 0.36 for rs365990 and was consistent with 1000Genomes Project's data in the European population – 0.34. The G allele (risk allele) associates with increased heart rate. Therefore, the genotype by G allele may be an additional factor to determine cardiovascular risk.