Determination of flavonoids and anthocyanins in Nitraria tangutorum by high performance liquid chromatography coupled with tandem mass spectrometry
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582.746.11:581.19:54.07 (1)
Botanică sistematică (850)
Fiziologia plantelor (522)
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GAO, Zhe Zhe, WANG, Ying-Chun, CHANG, Yan-Xu. Determination of flavonoids and anthocyanins in Nitraria tangutorum by high performance liquid chromatography coupled with tandem mass spectrometry. In: International Congress of Geneticists and Breeders from the Republic of Moldova, Ed. 11, 15-16 iunie 2021, Chişinău. Chișinău, Republica Moldova: Centrul Editorial-Poligrafic al Universităţii de Stat din Moldova, 2021, Ediția 11, p. 25. ISBN 978-9975-933-56-8. DOI: https://doi.org/10.53040/cga11.2021.009
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International Congress of Geneticists and Breeders from the Republic of Moldova
Ediția 11, 2021
Congresul "International Congress of Geneticists and Breeders from the Republic of Moldova"
11, Chişinău, Moldova, 15-16 iunie 2021

Determination of flavonoids and anthocyanins in Nitraria tangutorum by high performance liquid chromatography coupled with tandem mass spectrometry

DOI: https://doi.org/10.53040/cga11.2021.009
CZU: 582.746.11:581.19:54.07

Pag. 25-25

Gao Zhe Zhe, Wang Ying-Chun, Chang Yan-Xu
 
Inner Mongolia Agricultural University, China
 
Disponibil în IBN: 14 iunie 2021


Rezumat

Using high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-MSn) method, qualitative and quantitative analysis of flavonoids of stems, leaves, fruits and seeds, and anthocyanidin of fresh fruits in Nitraria tangutorum were performed. A total of 14 flavonoid components were identified from the seeds of N. tangutorum including three quercetin derivatives, three kaempferol derivatives, and eight isorhamnetin derivatives. A total of 12, 10, and 7 flavonoid components were identified from leaves, stems, and fruits of N. tangutorum, respectively; all were present in seeds also. The total content of flavonoids in leaves was the highest, up to 42.43 mg/gdry weight. A total of 12 anthocyanidin components were identified from the fresh fruits of N. tangutorum, belonging to five anthocyanidin. The total content of anthocyanidin in fresh fruits was up to 45.83 mg/100 g fresh weight, of which the acylated anthocyanidin accounted for 65.7%. The HPLC-DAD-MSn method can be operated easily, rapidly, and accurately, and is feasible for qualitative and quantitative analysis of flavone glycosides in N. tangutorum.