Ethylene glycol ethers, especially 2-ethoxyethanol (EE) and 2-butoxyethanol (BE) have an amphiphilic structure, with the hydrophilic part interacting with water, and the lipophilic part interacting with organic solvents, which explains their solubility in both water and organic solvents such as oils. Due to this characteristic, EE and BE are widely used in industry as ingredients of paints, inks, water-based cleaners, adhesives, detergents, cosmetics, cutting fluids, agrochemicals, jet fuels and hydraulic brake fluids [1,2]. The most promising method of cleaning aqueous solutions from these pollutants is their microbial utilization. The aim of our work was screening of potential glycol ester degrading microorganisms. The strains were isolated from various natural sources and deposited in specialized collection of microbial xenobiotic decomposers of the Laboratory of Environmental Biotechnology of the Institute of Microbiology, National Academy of Science of the Republic of Belarus. Hence, 68 isolates capable of using EE and BE as the sole source of carbon in concentrations up to 0.5% were isolated. Primary study of destructive ability by auxanographic method allowed selecting 17 isolates that showed good growth on all studied toxicants at 1% concentration. Among the collection strains, 15 bacterial cultures using glycol ethers in concentrations up to 1% were selected. The strains were cultured on E-8 medium (NaCl 0.5 g/L, MgSO4×7H2O 0.8 g/L, KH2PO4 0.7 g/L, (NH4)2HPO4 1.3 g/L, agar 22 g/L) with 0.2%, 1% and 2% of 2-butoxyethanol and 2-ethoxyethanol as the only source of carbon [3]. According to the results of the initial screening, we have shown the potential biodegradability of glycol ethers such as 2-ethoxyethanol and 2-butoxyethanol in various concentrations (0,1-2,0%). The following strains showed destructive activity on E-8 medium with 2-butoxyethanol as main source of carbon: Rhodococcus sp. 200N, R. ruber H2004, R. erythropolis 37F, R. erythropolis 70F, R. ruber 2B, Rhodococcus sp. R1, R. opacus 31D. These strains were characterized by active growth on E-8 medium at concentrations of 0.2%, 1% and 2% of 2butoxyethanol. The highest activity on E-8 medium was shown by R. wratislaviensis G13 on all investigated concentrations of 2-ethoxyethanol and R. ruber H2004 also on both concentrations of toxicant. R. erythropolis 70F, R. opacus 31D, R. ruber R1 were characterized by average growth activity on E-8 medium with 1% of 2-ethoxyethanol. The results obtained above suggest that these toxicants can be biodegraded by microorganisms of the genus Rhodococcus. Further studies will include the adaptation of selected microorganisms to higher concentration of toxicants. It seems promising to consider these strains of microorganisms as components of a biopreparation for treating wastewater containing glycol ethers. Currently, there is no reliable information on the degradation pathways of glycol esters among the microorganisms of this genus, which is a promising direction in the study of microorganisms.