Pseudomonas chlororaphis subsp. aurantiaca bacteria are producers of various biologically active substances, including products of the aromatic nature of the shikimate pathway. The shikimate pathway is a metabolic pathway that leads to the formation of chorismate, a precursor of essential amino acids, as well as siderophores, quinones, pigments, and phenazine compounds. Phenazines are a large group of nitrogen-containing heterocyclic compounds whose properties are determined by the presence and location of various functional groups in these compounds. They are used in agriculture (as antimicrobial agents), medicine (as antifungal and antitumor agents). Due to their ability to transfer electrons, the use of phenazines in biosensor technologies is promising. Regulation of the flow of metabolites along the shikimate pathway is carried out due to the products of seven genes phzABCDEFG of the phenazine operon (phz operon). Therefore, it is important to understand at what stage of bacterial growth (exponential phase or stationary phase) and at which genes of this operon are expressed at the highest level to have a possibility to influence on production of primary and secondary metabolites of shikimate pathway. In order to monitor the expression of the genes of the phenazine operon, as well as the gene encoding DAHP-synthase type II, located outside the operon in dynamics, three time intervals were selected for the exponential phase of bacterial growth (12, 18 and 24 hours) and two ones for the stationary phase of bacterial growth (4.5 days and 6 days). This experiment was conducted with P. chlororaphis subsp. aurantiaca B-162 bacteria that were cultivated on minimal medium (M9) and production media. Unique primers were developed to each gene of phz operon. PCR analysis was conducted with cDNA of each sample as matrix. In the case of Pseudomonas bacteria that were cultivated on production media, the expression level of the phenazine operon genes phzABCDE gradually increased from the initial stage of life cycle and reached the highest level on the 6th day of cultivation. Expression level of genes phzF and phzG showed the same trend, but their level is was lower than for the other genes. In sample of the 6th day of cultivation, PCR-products concentration of all phz genes was almost equal. This can be explained by participation of PhzF and PhzG at the later stages of secondary metabolite biosynthesis, while other proteins also catalyze reactions of primary metabolite biosynthesis. In the case of bacteria that were cultivated on minimal medium (M9), the genes of phz operon phzABCDEFG were expressed at the highest level from 12 to 24 hours. The expression level of genes phzF and phzG was lower than for the other genes of operon. Then, the expression level reduced and showed an upward trend on the 6th day of cultivation. Minimal media are not suitable for the production of secondary metabolites such as phenazine compounds. That is why, the highest level of phz operon expression was seen in the exponential phase of bacterial growth, when products of these genes catalyze reactions of primary metabolites such as aromatic amino acids. The resumption of operon expression on the 6th day of cultivation may indicate the participation of enzymes encoded in phz operon in some secondary metabolite biosynthesis, but not at the same level as on production media.