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573.6:543.9:582.232 (1) |
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SM ISO690:2012 DJUR (MAXACOVA), Svetlana, TAȘCA, Ion, CEPOI, Liliana, RUDIK, V.. Estimation of malondialdehyde content in Arthrospira platensis Cnmn-cb-11 cultivated in the presence of inorganic selenium (IV) compounds. In: Biotehnologii moderne - soluții pentru provocările lumii contemporane, 20-21 mai 2021, Chişinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2021, p. 56. ISBN 978-9975-3498-7-1. DOI: https://doi.org/10.52757/imb21.022 |
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Biotehnologii moderne - soluții pentru provocările lumii contemporane 2021 | |||||
Simpozionul "Simpozion ştiinţific naţional cu participare internaţională: " Chişinău, Moldova, 20-21 mai 2021 | |||||
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DOI: https://doi.org/10.52757/imb21.022 | |||||
CZU: 573.6:543.9:582.232 | |||||
Pag. 56-56 | |||||
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Accumulation of lipid peroxidation products in spirulina biomass may reduce its biological value and safety as a raw material for the production of various preparations. Malondialdehyde (MDA) has been recognized as an important lipid peroxidation indicator. Quantification of malondialdehyde was used to assess the level of oxidative stress induced in spirulina culture as a result of changes in cultivation conditions. In this study, some inorganic selenium (IV) compounds were used to cultivate spirulina, in order to obtain biomass with biotransformed content of this bioelement. MDA content was determined as a marker of possible toxicity of the compounds of selenium/selenium accumulated in spirulina culture. It was found that sodium selenite Na2SeO3 and ammonium selenite (NH4)2SeO3 had practically no effect on the accumulation of free radicals and lipid oxidation (Figure 1).figureFig.1. Malondialdehyde content in biomass of A. platensis CNMN-CB-11 cultivated in the presence of inorganic selenium (IV) compounds. The change in MDA content upon the addition of zinc selenite and cobalt selenite compounds was not determined by the supplemented concentration and did not depend on the amount of accumulated selenium (52,64 mg% for ZnSeO3 and 22,71 mg% for CoSeO3). In case of supplementation with Fe2Se3O9∙6H2O and GeSe2 in the presence of which the highest amount of selenium was accumulated in spirulina biomass, the MDA level has not changed substantially compared to control. Thus, the data revealed no significant increase in the MDA level, even at the highest quantities of accumulated selenium - in the case of ferric selenite hexahydrate and germanium selenide. These results suggest that cyanobacterial defense mechanisms against oxidative damage pose a biochemical barrier in response to inorganic selenium (IV) compounds. Spirulina responded by constant lipid synthesis, which made it possible to maintain membrane fluidity and cellular viability. The evidence supporting this claim was also the large amount of biomass produced or at the control level of spirulina culture. Therefore, the tested selenium compounds were not toxic to Arthrospira platensis CNMNCB11 within the limits of applied concentrations, while the vital processes were not interrupted and the balance of its biochemical composition was not disturbed. |
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