Articolul precedent |
Articolul urmator |
320 4 |
Ultima descărcare din IBN: 2023-11-13 21:41 |
SM ISO690:2012 MAZUR, Ecaterina, UNCU, Livia, SCHMID, Martin. Aplicarea cromatografiei de lichide de înaltă presiune în evaluarea compatibilităţii orotatului de potasiu şi a spironolactonei. In: Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, 21-23 octombrie 2020, Chişinău. Chişinău: USMF, 2020, p. 668. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu” 2020 | ||||||
Congresul "Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”" Chişinău, Moldova, 21-23 octombrie 2020 | ||||||
|
||||||
Pag. 668-668 | ||||||
|
||||||
Descarcă PDF | ||||||
Rezumat | ||||||
Background. Compatibility determination of active substances in fixed-dose drug combinations is an indispensable step in their elaboration. High-performance liquid chromatography (HPLC) provides information on possible interactions between the components and the occurrence of degradation products. Objective of the study. To investigate the compatibility of potassium orotate with spironolactone in combination by HPLC method. Material and Methods. Liquid Chromatograph Agilent 1100 with UV-VIS detector and RP-18 reverse column (250*4 mm, 5 μm), mobile phase of acetonitrile:phosphate buffer solution (50:50), flow rate 1,5 ml/min, volume of the sample 20 μl, temperature 40°C; potassium orotate and spironolactone substances (Sigma Aldrich, USA). Results. Due to developed method it was carried out the separation and simultaneous determination of the both substances in the mechanical mixture and evaluation of their concentrations. For spironolactone: retention time 6,42 min, concentration 98,1%, RSD=0,21, E=1,2%, symmetry 0,95; for potassium orotate: retention time 1,25 min, concentration 72,8%, RSD=0.12, E=37,3%, symmetry 0,65. The formation of new products in the mixture was not found (lack of additional peaks in the chromatograms). Conclusion. The studied substances are compatible. Studies will be performed by other methods (DSC, FT-IF Spectrophotometry) to confirm the obtained result. |
||||||
Cuvinte-cheie HPLC, Combination, potassium orotate, spironolactone, HPLC, combinație, orotat de potasiu, spironolactonă |
||||||
|