Currently, in the food industry, it is given attention to processing autochthonous walnuts in order to obtain quality products with high nutritional value. Walnuts present a valuable raw material considering the content of unsaturated fatty acids, saturated acids, vitamins, antioxidants, minerals. During the storage, the quality of walnut kernels decreases and this process alters the organoleptic and physicochemical properties. This paper shows the changes of the chemical composition of walnut (Juglans regia L) grown in Moldova during the storage. There were estimated the following indicators: acidity, peroxide index, water activity. The study was conducted on autochthonous nuts which were stored for 1 month, 1 and 2 years.