Among the representatives of oil crops, fruits of walnut (Juglans regia L.) are differ by the richest in lipid content 65...75%, proteins 15…17%, vitamins, minerals, polyphenolic compounds etc. Processing of nuts along with the organization of industrial production of walnut oil is among the most promising directions for the national economy of the Republic of Moldova. Walnut oil is a product of an exceptionally high nutritional value, with high content of biologically active substances, including essential unsaturated fatty acids, the ω-3 and ω-6. During storage, walnut oil is extremely unstable, subject to physical and chemical changes with the accumulation of primary and secondary oxidation compounds.