The aim of this study was an analysis of the sediment quality of the lake Beleu, natural reserve of Republic of Moldova in Low Prut River region. This area is under intensive anthropogenic impact from petrol mining, agriculture and transport. The sediment samples were taken from the area of petrol mining and from other territory of Beleu lake. The analysis of POPs, PAHs substances and heavy metals in sediment samples was carried out for the evaluation of the chemical status of this water body. The analysis was made by Gas Chromatography and Atomic Absorption Spectrophotometry methods in accredited laboratory. The total PAHs concentration in water samples was in the range from detection limit (<0.001) to 0.117 μg/L and Benzo[a]pyrene concentration was below the detection limit of 0.001 μg/L. The total PAHs value in sediment samples was in the interval 0.022 – 1.050 mg/kg. The total POPs concentration in water samples was in the range from detection limit 0.001 to 0.053 μg/L. The total POPs concentration in sediment samples was in the interval from 0.030 to 1.448 mg/kg. About 20 % of sediment samples had a total POPs value higher than 0.10 mg/kg. The principal POPs were organochlorine pesticides in 70 % of sediment samples and PCBs for 30 % of sediment samples. The heavy metal concentration in sediment and water samples was at the background level or below of the detection limit. The conclusion is that the Beleu natural lake is under strong anthropogenic impact from different pollution sources including POPs polluted sites from past pesticide storages. The specific sediment monitoring is required for the more correct assessment of the status of this water body. Acknowledgement: We acknowledge the funding from ENPI project code MIS ETC 1676 (2013-2015) and ENI project eMS code BSB27 (2018-2021).