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SM ISO690:2012 ZAMORZAEVA-ORLEANSCAIA, Irina, BAHŞIEV, Aighiuni, TUMANOVA, Lidia. Optimal timing and sampling for a reliable assessment of the resistance of tomato varieties to ‘candidatus phytoplasma solani’. In: Phytopathogenic Mollicutes, 2019, nr. 1(9), pp. 159-160. ISSN 2249-4669. DOI: https://doi.org/10.5958/2249-4677.2019.00080.X |
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Phytopathogenic Mollicutes | |||||
Numărul 1(9) / 2019 / ISSN 2249-4669 /ISSNe 2249-4677 | |||||
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DOI: https://doi.org/10.5958/2249-4677.2019.00080.X | |||||
Pag. 159-160 | |||||
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Rezumat | |||||
The difference in the resistance of two Moldovan tomato varieties to ‘Candidatus Phytoplasma solani’ was determined using nested-PCR analysis on DNA extracted from individual plants by the boiling express method. Two important conclusions were drawn as a result of the study. Firstly, the period of mass fruit ripening is the most suitable period for the molecular detection of phytoplasma presence in tomato plants for assessing the resistance of varieties. Secondly, a total of 11–12 plants of each variety is sufficient for obtaining statistically significant results at this period of the tomato growth. |
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Cuvinte-cheie Molecular detection, Optimal timing and sampling, susceptibility, Tomato‘, Candidatus Phytoplasma solani’ |
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