The conservation of biological diversity is receiving great attention. Its reduction threatens humanity with an irreplaceable loss of many resources, degradation and destruction of the biosphere. The depletion of animal genetic resources can cause a variety of negative consequences: firstly, the efficiency of breeding work will decrease significantly; secondly, already existing animal breeds will not be able to successfully resist constantly evolving pathogens of diseases and will become an easy victim of epizootics; thirdly, valuable genetic material for studying the evolution of animals will be lost. To preserve biodiversity genetic resources, a non-alternative method is cryopreservation using the vitrification method.  Vitrification refers to the transition of a liquid into a solid state, caused not by crystallization, but by an extreme increase in viscosity during cooling. Vitrification fluid consists of a mixture of highly concentrated penetrating cryoprotectant (acetamide, propylene glycol, glycerol, ethylene glycol) and non-penetrating cryoprotectant (polyethylene glycol, ficoll, sucrose) in a buffered saline solution. The concentration of the cryoprotectant is so high that with very rapid cooling to -196 °C, the viscosity greatly increases.  This is a modern method of cryopreservation of reproductive cells that provides a higher level of their survival after freezing due to the use of highly concentrated solutions, as well as small volumes and limited time. This avoids the formation of ice crystals inside the cell, which often causes its irreversible destruction.  Embryos can be frozen by vitrification. Before freezing, they are kept in vitrifying solutions containing ethylene glycol, propanediol, sucrose, elglutamine. Cryopreservation is carried out by an open method. For thawing, frozen objects are immersed in a vitrifying solution containing sucrose, glutamine for several minutes, followed by transfer to a washing solution.The transfer is carried out the next day after thawing, less often it can be allowed day to day. Using this technology, it is possible to obtain more than 90% of human embryos that have retained the normal morphology of all blastomeres and non-detecting signs of negative effects. Vitrification now occupies a dominant position in the field of activity of embryologists and at present there is no particular alternative to replace vitrification yet in this regard. However, transplantation of devitrified embryos recorded about 30% of clinical pregnancies. This indicates that, despite the prospect of vitrification, this method needs substantial improvement, after which it can find wide application in the practice of human and animal reproduction.  Vitrified objects can be used to maintain the number of bioorganisms, for which genetic banks are called upon to play a large role. The creation of such banks will save genetic information for the following groups of animals and plants: 1) endangered and rare species; 2) promising species for domestication, breeding, medical and other needs of the national economy; 3) representatives of native breeds of animals and plant varieties; 4) outstanding individuals.  Thus, at present there are significant successes in solving the problems of reproduction of animals using vitrified material. However, further trends in the development of research in the field of of cryopreservation should be aimed at a deeper study of the mechanisms of cryodamage and cryoprotection of reproductive cells; continuation of research to identify the relationship between the chemical structure, physico-chemical properties and toxicity of cryoprotectants; the creation of new, more effective cryotechnologies.