| SM ISO690:2012|
ELENCIUC, Daniela; BULIMAGA, Valentina; EFREMOVA, Nadejda; ZOSIM, Liliana; BATYR, Ludmila. The application of some coordination compounds in regulation of the content of SOD in Spirulina platensis biomass. In: Ştiinţele vieţii în dialogul generațiilor: conexiuni dintre mediul academic, universitar și de afaceri. 21-22 octombrie 2019, Chişinău. Chișinău, Republica Moldova: Tipogr. "Biotehdesign", 2019, pp. 90-91.
|Ştiinţele vieţii în dialogul generațiilor: conexiuni dintre mediul academic, universitar și de afaceri 2019|
Conferința "Life sciences in the dialogue of generations: connections between universities, academia and business community" |
ed. IV, Chişinău, Moldova, 21-22 octombrie 2019
Investigation of non-traditional sources of bioactive substances is one of the current directions of biotechnology development in many countries worldwide. Cyanobacteria Spirulina platensis is widely explored and used in recent decades as a source of valuable biologically active substances [1, 5.]. An important role in neutralizing the harmful effects of oxidative stress have the enzime superoxiddismutase (SOD), that it is present, also, in the biomass of cyanobacteria Spirulina platensis. The influence of vanadium compounds on photosynthesis in cyanobacteria hasn’t been yet studied completely. Pharmacological uses of vanadium include lowering of cholesterol, triglycerides and glucose levels . Vanadium also possesses anti-carcinogenic and anti-diabetic properties . Another trace element - cobalt has important biological significance, ions of which are actively involved in the reactions of oxidation and reduction, have a positive influence on the processes of cell respiration and metabolism, as well as on the biosynthesis of phycobiliproteins and nucleic acids . The aim of this investigation presents a study of possibility of utilization of some coordination compounds of V(IV) and Co(III) as regulators of the content of superoxide dismutase in the biomass of cyanobacteria Spirulina platensis. The high degree of biological activity of the cyanobacteria Spirulina platensis is caused mainly by the presence of antioxidants in the composition of its biomass. It was established a weak inhibitory effect of the two studied compounds: [(VO)2(2PyTCH)]SO4•4H2O in concentrations within 5 to 25 mg/l and Na[Co(DH)2(NO2)2] (10-25mg/l) on the productivity of spirulina. In the case of utilization of the other compounds, productivity values are within the reference sample, except for compounds [Co(L-H)En]·3H2O and Na[Co(DH)2(NO2)2], which contribute to increase of productivity by 11-18% compared to the reference sample in the concentration of 5 mg/l. The determination of activity of superoxiddismutase (SOD) in obtained extracts from spirulina biomass allowed to establish a positive effect of coordination compounds of Co (III) on the activity of this enzyme. Maximum increase of superoxiddismutase activity in the biomass of spirulina (by 38% compared to the reference sample) was established in the case of utilization of the compound [Co(LH)En]·3H2O in the concentration of 15mg/l. The significant increase of SOD activity (by 32-36% compared to the reference sample) is registered in the case of administration of the compound Na[Co(DH)2(NO2)2] in the concentration range of 15-25mg/l. The present study reveals that obtained biomass of cyanobacteria Spirulina platensis with high content of SOD can be used for the elaboration of medical remedies for prophylaxis and treatment of diseases, caused by the negative effect of oxidative stress on live organisms and, also, for the elaboration of cosmetic preparations for the prevention of premature skin aging, protection against solar radiation and treatment of skin diseases.