Study of the wild, spontaneous hemp forms and local populations regarding the content of THC and their use in the breeding process of the monoecious hemp
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GĂUCĂ, Constantin; POPA, Diana; BUBURUZ, AlexandraAndreea; DRUŢU, AdinaCătălina; POCHISCANU, Simona-Florina; NAIE, Margareta; LĂZĂROIU, Daniel. Study of the wild, spontaneous hemp forms and local populations regarding the content of THC and their use in the breeding process of the monoecious hemp. In: Conservation of plant diversity. Ediția 4, 28-30 septembrie 2015, Chișinău. Chișinău: Gradina Botanica (Institut), 2015, p. 70. ISBN 978-9975-3036-8-2.
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Conservation of plant diversity
Ediția 4, 2015
Simpozionul "Conservation of plant diversity"
4, Chișinău, Moldova, 28-30 septembrie 2015

Study of the wild, spontaneous hemp forms and local populations regarding the content of THC and their use in the breeding process of the monoecious hemp

Pag. 70-70

Găucă Constantin, Popa Diana, Buburuz AlexandraAndreea, Druţu AdinaCătălina, Pochiscanu Simona-Florina, Naie Margareta, Lăzăroiu Daniel
Agricultural Research and Development Station Secuieni
Disponibil în IBN: 14 mai 2019

monoecious hemp, chromatographic method, THC content


Reducing of the cannabinoid content, and maintaining the monoecious character in the new creations, under the conditions of existing in the research area of the dioecious hemp forms, spontaneous, local populations or derived from former cultivated varieties with dominant characters on the monoecious and THC content has proven very difficult. The pronounced allogamy of hemp, high pollen viability and spread resistance by air currents led us to become involved in a study regarding the spreading of these forms (Ceapoiu, 1976). The 2009 collecting expedition in the Subcarpathian area and northern area of Moldova resulted in the collection of seven resources considered populations, of which only one can be characterized as such and useful to the improvement process, the rest being sourced from the varieties recently found in crop.  MATERIAL AND METHOD The identification of the distribution areas and the collection of new genetic resources from the spontaneous and cultivated flora was carried out by organizing two scientific expeditions in 1991 and 1992, and for the cultivated forms which have become spontaneous and local populations, in 1996 and  2009. The seed collection was made on a large number of plants in mixture, for the coverage of maximum potential variability of the area, and also on individual plants analyzed for highlighting the genetic and phenotypic characters in the experimental field. The cannabinoid content was determined orientatively through the colorimetric method, which consists of a color scaler, with grades from 1 to 10, corresponding to the 0.00 – 1.0% THC content. The maximum grade was awarded to the maximum color intensity with shades of purple. The method has been approved and the improvement process regarding the reduced cannabinoid content gave satisfactions, by obtaining Dacia Secuieni and Secuieni Jubileu varieties, with an unidentifiable content through the quantitative chromatographic method. RESULTS AND DISCUSSIONS The expeditions of identifying the spontaneous hemp forms had as purpose to establish their spread in Romania, the taxonomic classification and their influence on the cultivated forms. The pronounced alogamy and the dominant dioecious character caused the formation of genotypes with adaptability to environmental stress conditions, the soil drought and atmospheric heat, which were consolidated as wild and primitive populations that occupy vast areas in some parts of the country.  The spontanea sp. ramosa var. has specific characters for the area between the Danube and 44o50’latitude, and as eastern longitude between 22o30’ and 28o30’. The intermedia var. is specific for some areas from central and northern Dobrogea, the northern part of Baragan and the central and eastern area of Moldova, until at 46o10’ latitude. The spontaneous ramosa and intermedia varieties have a THC content noted with 9 and 10, content that decreases from south to north, and at local populations, the content is variable, noted with 5 and 6, the inclusion in the improvement process requiring a reduction of THC content below 0.2%. Reducing the cannabinoid content in the improvement process was done by direct hybridization, the dioecious form with high content – maternal genitor and the monoecious lines or varieties with low content as paternal genitor. Through backcrossing B1, B2 and individual selection, the content was reduced below 0.01% or even unidentifiable through the chromatographic methods at the new creations of ARDS Secuieni, the cultivars Dacia Secuieni for fiber and Secuieni Jubileu for seed production.