Some biologal and phytochemical aspects of  Monarda fistulosa l. species from center of medicinal plant cultivation of USMF "Nicolae Testemitanu"
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2023-02-22 16:47
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CALALB, Tatiana, LECA, Ion. Some biologal and phytochemical aspects of  Monarda fistulosa l. species from center of medicinal plant cultivation of USMF "Nicolae Testemitanu". In: Conservation of plant diversity, Ed. 4, 28-30 septembrie 2015, Chișinău. Chișinău: Gradina Botanica (Institut), 2015, Ediția 4, p. 55. ISBN 978-9975-3036-8-2.
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Conservation of plant diversity
Ediția 4, 2015
Simpozionul "Conservation of plant diversity"
4, Chișinău, Moldova, 28-30 septembrie 2015

Some biologal and phytochemical aspects of  Monarda fistulosa l. species from center of medicinal plant cultivation of USMF "Nicolae Testemitanu"


Pag. 55-55

Calalb Tatiana, Leca Ion
 
”Nicolae Testemițanu” State University of Medicine and Pharmacy
 
 
Disponibil în IBN: 14 mai 2019


Cuvinte-cheie
Monarda fistulosa, biometry, microscopy, flavonoids, tannins


Teza

Monarda fistulosa L., the common name – wild bergamot is known in spontaneous flora of North America (from Quebec to the Northwest territories and British Columbia, South to Georgia, Texas, Arizona, Idaho, and Washington) and today is cultivated in American, European and Asian countries as ornamental, aromatic, culinary and medicinal plant. The wild bergamot vegetable drug is characterized by especially volatile oils content, flavonoids and tannins with multiple therapeutic effects [1, 2]. That is why M.fistulosa was introduced in collection of plants of Center of Medicinal Plant Cultivation of USMF "Nicolae Testemitanu" in 2009 and it has been grown as three chemotypes – violet (with the highest content of corvacrol), purple (with thymol) and white (mixed – corvacrol and thymol) [1]. In the wild bergamot plantation, were made phenological observations, biometric measurements to plants and collecting of vegetable products (Monardae folia, M. flores and M. herba) for microscopic and phytochemical analyses. The importance of this study is to determine which of the three chemotypes (white, violet and purple) is most suitable to be cultivated in the pedoclimatic conditions of the Republic of Moldova and to elucidate the best vegetable product of M.fistulosa L. as a source of phenolic compounds, also to establish the microscopic indices to identify the vegetable products.  Wild bergamot vegetable products (leaves, flowers, aerial parts) were used for microscopic analyses, qualitative study of flavonoids and tannins by specific colour or sedimentation reactions and thin layer cromatography, dosing of flavonoids – spectrophotometrically and tannins – titrimetrically. The highest biometric values (plant height, number or branches, number of flower clusters, leaf size) were referred for violet, then purple and white chemotypes of wild bergamot. Microscopic indices for all vegetable products are: 2 types of secretory hairs, one – short stipe and 8-cellular gland with brownish content and other – 2-3 cells stipe and unicellular gland; dens uni- and multicellular protective trichomes, especially on lower surface of the leaves and bracts. The highest frequency of secretory hairs with 8-cellular gland was mentioned on leaves, bracts and petals.  Qualitative analysis of flavonoids shows the presence of flavonoid constituents with varying degrees of intensity: in leaves – chalcones and aurons (+++), hydroxyflavone (+); in aerial parts – chalcones and aurons (+), hydroxyflavone (++) and anthocyanins (++); but in flowers, only – hydroxyflavone (+++) and anthocyanins (+++).  In flowers, it was found the highest content of flavonoids – 2.87 %, followed by aerial parts – 2.79% and in the leaves – the lowest content (2.73%). Screening of colour and sedimentation reactions in the all extracts denotes only the presence of condensed tannins with different intensity in all analyzed specimens.  Dosage of tannins by titrimetric method showed that the leaves have higher content of tannins – 17.2%, than the flowers – 12.4% and the lowest is in the aerial parts – 9.0% of M.fistulosa plant. Flavonoids and tannins content of vegetable drugs of M. fistulosa grown in pedoclimatic conditions of the Republic of Moldova varies: aerial parts with the highest content of both (flavonoids and tannins), while flowers containing a maximum of flavonoids and minimum of tannins, and leaves – contrary. The importance of this study is that species Monarda fistulosa L. is suitable to be cultivated in the pedoclimatic conditions of the Republic of Moldova as a new medicinal plant for our region with rich specter of active principles.