Actinomycetes of genus Streptomyces are important producers of biologically active substances with antifungal and growth stimulating properties. The aim of our study was to isolate and identify such important strains from soil samples collected in the central part of R. Moldova. Tomato seeds (cv. Fakel) were soaked for 4 days at 26 ºC in aqueous solutions of exometabolites (EMs) of different strains of Streptomyces at 0.5 and 1.0% concentrations, and grown for 4 days to assess percent seed germination and root length. Seed germination of tomato seeds treated with EMs of Streptomyces sp.11 and 33 at 1.0% concentration was significantly higher than that at 0.5% concentration. No significant difference was observed in the per cent seed germination between the untreated control and Streptomyces sp.47. A higher seed germination in comparison to the control and 1.0% concentration was observed at 0.5% EMs concentration for Streptomyces sp. 49 and 154 (+ 11.3%). Five on six tested Streptomyces strains showed a positive effect on root development in comparison to the control. EMs of Streptomyces sp.11, 33 and 76 had a positive effect at 1% concentration. In particular EMs of Streptomyces sp.11 and 76 at the higher and lower concentration significantly increased root length in comparison to the control by 82.0 and 82.6 %, and 51.5 and 73.1 %, respectively. However the highest root length increase effect was obtained by EM of Streptomyces sp.49 at 0.5 and 1.0% concentrations. At these concentrations, in comparison to the untreated control, an increase was recorded of 89.2 % and 74.3%, respectively. On the contrary for EM of Streptomyces sp.154 root length was increased of 14.4 % at 0.5% concentration and reduced of 26.9 % at 1.0% concentration. The antifungal activity of the different strains of streptomycetes was detected agains Аlternaria alternata, Аspergillus flavus, А. niger, Botrytis cinerea, Fusarium graminearum, F. oxysporum, F. solani, Rhizoctonia solani, Sclerotinia sclerotiorum and Thielaviopsis basicola using the method of disc-diffusion. A complete growth inhibition of A.alternata was noted by Streptomyces sp.10, 23, 33, and 37. Streptomyces sp. 10 was also able to inhibit growth of B. cinerea and F. graminearum. S. sclerotiorum was completely suppressed by Streptomyces sp. 9 and 42. On the base of diameter of zones of growth inhibition which ranged between 24.0 and 34.0 mm, antifungal activity was also recorded for Streptomyces sp.9 (all tested phytopathogenic fungi), 12 (A. alternata and T. basicola) , 17 (A. alternata), 33 and 37 (B. cinerea and F. graminearum), 66 (А. alternata, А. flavus and S. sclerotiorum) and 193 (S. sclerotiorum).