Influence of Z O nanoparticles on the biomass production and catalase activity of the yeast Saccharomyces Cerevisiae CNMN-Y-20
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CHISELIŢA, Natalia. Influence of Z O nanoparticles on the biomass production and catalase activity of the yeast Saccharomyces Cerevisiae CNMN-Y-20. In: Microbial Biotechnology, 12-13 octombrie 2016, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2016, Ediția 3, p. 164.
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Microbial Biotechnology
Ediția 3, 2016
Conferința "Microbial Biotechnology"
Chișinău, Moldova, 12-13 octombrie 2016

Influence of Z O nanoparticles on the biomass production and catalase activity of the yeast Saccharomyces Cerevisiae CNMN-Y-20


Pag. 164-164

Chiseliţa Natalia
 
Institutul de Microbiologie şi Biotehnologie al AŞM
 
Disponibil în IBN: 15 martie 2019



Teza

Currently it is known that nanoparticles have both, toxic as well as stimulating action on microorganisms: that occurs depending on their composition, size and concentration. Among the various metal oxide nanoparticles most commonly used are zinc oxide nanoparticles [2]. Catalase activity is one of the parameters indicating the cell state. The basic catalase function in living cells is to reduce free radicals, H2O2 and active oxygen forms, that leads to cell membrane lipid oxidation and cell membrane destruction in adverse conditions [1]. In this context, the aim of the research was to evaluate the influence of ZnO nanoparticles on biomass production and catalase activity of Saccharomyces cerevisiae CNMN-Y-20 yeast strain, an active producer of β-glucans, deposited in the National Collection of Nonpathogenic Microorganisms. For the research ZnO nanoparticles with size of 30 nm, dispersed in Poly (N-vinylpyrrolidon) in concentrations of 0,5; 1,0; 5,0; 10,0 and 15 mg/L , and YPD medium were used. The nanoparticles were synthesized by the researchers of the Institute of Electronic Engineering and Nanotechnology of ASM. As a control was examined variant without nanoparticles. The yeast biomass was collected after 120 hours of cultivation. The catalase activity was measured after 120 hours of cultivation, also. Thus, the research results have demonstrated that the cell biomass cultivated in the presence of ZnO nanoparticles, was reduced insignificantly (by 3,2 to 5,8% compared to the control sample) irrespective of the used concentration. Assessing the catalase activity in experimental samples, it has been established that in all variant, filled with ZnO nanoparticles, it was increased by up to 29% compared to the control sample, that indicates the stress state of cells, induced by the presence of nanoparticles in the cultivation medium. Maximum effect was recorded at the use of the concentration of 5 mg/L of nanoparticles. The using of 10 and 15 mg/L concentrations leads to the decrease of catalase activity. This phenomenon can be explained by the fact that increasing of the nanoparticles concentration causes disequilibrium of the reactive oxygen production and the ability to detoxify the cellular components that in turn leads to decreasing of the catalase activity. The absence of catalase for neutralizing H2O2 can cause grave cell damage. Thus, we conclude that ZnO nanoparticles, 30 nm in size, represent a damage factor that can affect some cell components of S. cerevisiae CNMN-Y-20 strain.