In submerged culture processes, filamentous microorganisms typically have a complex morphology during their life cycle that is related to production performance – a relation that is of high interest for optimization process. The morphology is strongly influenced by process parameters, including mechanical stress due to stirring and aeration, pH level, composition of the culture medium (Nielsen et al., 1995; Papagianni, 2004; Bizukojc and Ledakowicz, 2010, Wucherpfennig, 2012), osmolality and the presence of solid microparticles (Krull et all, 2013; Kaup et all, 2008; Driouch et all, 2011). The aim of this review was to present the impact of nanoparticles on morphological engineering techniques in the cultivation of filamentous fungi. Under submerged cultivation on agarized media, it was revealed the ability of MgO, ZnO, MgO/ZnO, TiO2 and Fe3O4 nano-oxides with different physicochemical characteristics to induce morphological changes in mycelial fungi from the genera Fusarium, Trichoderma, Rhizopus – producers of exocellular hydrolases. The addition of 30 nm ZnO nanoparticles and 65-70 nm Fe3O4 nanoparticles, selected in prior researches as effective stimulators of exocellular protease biosynthesis in Fusarium gibbosum CNMN FD 12 and Trichoderma koningii CNMN FD 15 strains, resulted in appearing the colonies with distinct morphology, differing in size, color and mycelium structure on the agar medium. In order to enhance the biosynthetic activity of producer strains, it was revealed the opportunity to use as seed material cultures derived from morphologically modified colonies under the influence of nanoparticles. The utilization of morphologically modified colonies under the influence of ZnO and Fe3O4 nano-oxides as inoculum at submerged cultivation increased the activity of neutral proteases by 192.6% and 48.1% in Trichoderma koningii CNMN FD 15 and Fusarium gibbosum CNMN FD 12 strains, respectively. Fe3O4 nanoparticles measuring 70 nm did not change the morphology of Rhizopus arrhizus CNMN FD 03 culture. However, Fe3O4 nano-oxide exerted an essential positive influence on fungal metabolism, resulting in increasing the lipolytic activity by 248.8% (3.5 times) and appearing the biosynthesis absorption peak on the second day of cultivation, being with 24 hours earlier than the previous assessment model. Iron nano-oxide ensured the expansion of stationary growth phase of micromycete Rhizopus arrhizus CNMN FD 03. Moreover, stimulatory effect was significantly higher than control (with 45.8-248.8%) throughout the overall cultivation cycle (days 1-4).