Isolation of trifluralin degrading microbial consortium
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RASTIMEŞINA, Inna, POSTOLAKY, О., JOSAN (VORONA), Valentina, ŞAMUGHIA, Daria, STREAPAN, Nina, MAMALIGA, Vera, GUTSUL, Tatiana. Isolation of trifluralin degrading microbial consortium. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, p. 142. ISBN 978-9975-3178-8-7.
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Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

Isolation of trifluralin degrading microbial consortium


Pag. 142-142

Rastimeşina Inna1, Postolaky О.1, Josan (Vorona) Valentina1, Şamughia Daria1, Streapan Nina1, Mamaliga Vera1, Gutsul Tatiana2
 
1 Institute of Microbiology and Biotechnology ,
2 Institute of the Electronic Engineering and Nanotechnologies "D. Ghitu"
 
Disponibil în IBN: 21 februarie 2019



Teza

The genetic heterogeneity of microbial communities from pesticide-contaminated soil allows some microorganisms to adapt to changes in the characteristics of the energy substrate. The biochemical and genetic basis of microbial transformation of xenobiotics was linked to several genes/enzymes providing microorganism with the ability to degrade halogenated organic pesticides. Despite the high speed of reproduction of the microorganisms, the process of adaptation in the soil takes decades. Therefore, the most effective way of searching for the microorganism-destructor of xenobiotics is to isolate it from the natural environment and to adapt the microorganism to high concentrations of the toxicant. Due to the high toxicity of halogenated pesticides and, often, the presence of two or more contaminants in polluted soil, it is necessary to search for ways to optimize the biooxidation processes of such substrates. In this research, a technological scheme was developed for the process of isolation a microbial consortium involved in the degradation of herbicide trifluralin, using iron (II, III) oxide (Fe3O4 or magnetite) nanoparticles and zero-valent iron Fe(0) nanoparticles. The consortium of microorganisms was created on the basis of an accumulative culture obtained by inoculation of soil contaminated with pesticides into the PAS nutrient medium, pH varied from 5.0 to 6.5. The evolution of microbial consortium was observed through series of passages. Regulating the acidity of medium for accumulation culture, it is possible to control the development of a microbial consortium to the predominance of bacterial or fungal cultures. Depending on the acidity of the medium, trifluralin promoted an increase in the number of bacteria and a sharp increase in the number of micromycetes. A consequent increase in the concentration of trifluralin in the medium for accumulation culture has led to a reduction (depletion) in the species diversity of bacteria and micromycetes. An addition of Fe3O4 and Fe(0) nanoparticles to the medium restored the species diversity of these microorganisms. Every increase in the trifluralin concentration in the medium with acidity of pH 5.0 led to a gradual decrease in the number of bacteria, while the additon of Fe(0) nanoparticles in the same condition contributed to an increase in their numbers. The magnetite and zero-valent iron nanoparticles stimulated the growth and reproduction of micromycetes on the initial etapes of development of the consortium, when trifluralin concentration range was 100-200 mg/L. Then, when the concentration of trifluralin reached to 300-400 mg/L, the number of micromycetes did not depend on the acidity of medium, or on the presence of nanoparticles.