The In Vitro effect of the En- preparation on the pre-immune resistance indices in patients with chronic tonsillitis
Закрыть
Articolul precedent
Articolul urmator
950 0
SM ISO690:2012
DANILOV, Lucian, GHINDA, Serghei, CHIROŞCA, Valentina, ROTARU, Natalia, TROFIMCIUC, M., IASCHINA, Victoria. The In Vitro effect of the En- preparation on the pre-immune resistance indices in patients with chronic tonsillitis. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, pp. 49-50. ISBN 978-9975-3178-8-7.
EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core
Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

The In Vitro effect of the En- preparation on the pre-immune resistance indices in patients with chronic tonsillitis


Pag. 49-50

Danilov Lucian1, Ghinda Serghei2, Chiroşca Valentina2, Rotaru Natalia2, Trofimciuc M.1, Iaschina Victoria1
 
1 Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“,
2 IMSP Institutul de Ftiziopneumologie „Chiril Draganiuc“
 
Disponibil în IBN: 18 februarie 2019



Teza

Polyphenols (isolated from plants) are one of the types of antioxidants that protect cells of the human body from harmful reactions. Polyphenols are not only antioxidants, but also probiotics, i.e. they suppress the growth of the pathogenic microflora of the digestive tract, thereby promoting the vital activity of beneficial bacteria. The ongoing differentiation and specialization of immunology branches raise the today`s urgent problems - the need to develop a new direction that emerged at the junction of immunology, phytoimmunology and biotechnology – immune-biotechnology. The preparation EN is of interest, being prepared from walnut, which contains polyphenols up to 20-22%, free amino acids up to 0.92-1.02% (of which essential ones are up to 0.17- 0.20%, immunoactive ones being up to 0.20 -0.22%), iodine - up to 0.2 mg/l and iron - up to 0.4 mg / l. In this regard, we set the goal to investigate the in vitro effect of the EN preparation on neutrophil activity by nitroblue tetrazoliun (NBT) test. Material and methods. The study included 39 patients with chronic tonsillitis. The object of study was patients` neutrophils. A standard NBT test was performed, a suspension of leukocytes was poured into the control holes, while a leukocyte suspension and EN preparation diluted 1/128 was poured in the test holes. Discussion of the results. The NBT-test in the group of healthy children was 0.12 ± 0.003, which is significantly higher than in the 1 subgroup of patients with the stimulating effect (p<0.001) and significantly less than in the 3 subgroup of patients with a suppressive effect (p< 0.001). The NBT-test in the 2 subgroup of patients, where no modulating effect was found, was the same as in the healthy group. The NBT-test in the 1 subgroup, where the stimulating effect was noted, was initially significantly lower than in the 2 and 3 subgroups (p<0.001 in both cases), and under the influence of the EN preparation it increased to the level of healthy ones. The NBT-test in group 3, where the suppressive effect was noted, was initially significantly higher than in the 1 and 2 subgroups (p<0.001 in both cases), and under the influence of the EN preparation it decreased to the level not significantly different from the healthy ones. Thus, the EN preparation does not have a modulating effect on the NBT-test parameters close to those of healthy ones. EN has a stimulating effect on the NBT-test which is lower than in healthy people, and EN has a suppressive effect on the NBT-test which is higher than in healthy ones. Conclusion: the EN preparation showed a multidirectional effect; the EN preparation acted in a stimulating manner on low indices, in a suppressive manner on high indices and on indices close to normal - did not have any modulating effect. The EN preparation can be used to modulate the preimmune resistance indices in children with chronic tonsillitis aged 15 years and older.