| Conţinutul numărului revistei |
| Articolul precedent |
| Articolul urmator |
 662 1 |
| Ultima descărcare din IBN: 2018-10-29 04:11 |
 SM ISO690:2012HNDROULIS, Evangelia, MOVILĂ, Alexandru, SABHACHANDANI, Pooja, SARKAR, Saheli, COHEN, Noa, KAWAI, Toshihisa, KONRY, Tania. A droplet-merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli. In: Biotechnology and Bioengineering, 2017, vol. 114, pp. 705-709. ISSN 0006-3592. DOI: https://doi.org/10.1002/bit.26196 |
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  Biotechnology and Bioengineering |
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| Volumul 114 / 2017 / ISSN 0006-3592 | ||||||
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| DOI:https://doi.org/10.1002/bit.26196 | ||||||
| Pag. 705-709 | ||||||
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Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility and interaction within the confined space. Dynamic analysis of cellular heterogeneity in droplets has provided insights in various biological processes. Droplet manipulation methods such as fusion and fission make it possible to precisely regulate the localized environment of a cell in a droplet and deliver reagents as required. Droplet fusion strategies achieved by passive mechanisms preserve cell viability and are easier to fabricate and operate. Here, we present a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity. Biotechnol. Bioeng. 2017;114: 705–709. |
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| Cuvinte-cheie Bacteria |
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