Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
1141 8 |
Ultima descărcare din IBN: 2023-06-16 15:12 |
Căutarea după subiecte similare conform CZU |
618.3-007-06-036.17-07-053.1 (1) |
Mедицинские науки (11142) |
SM ISO690:2012 SINIŢÎNA, Lilia, DAVID, Valeriu, PETROVICI, Virgil, SAMCIUC, Stefan, BOIAN, Veaceslav, BOIAN, Gavril. Evaluarea particularităţilor morfopatologice prin metode clasice şi moleculare la copii cu vicii congenitale intestinale. In: Buletin de Perinatologie, 2016, nr. 1(69), pp. 38-43. ISSN 1810-5289. |
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Buletin de Perinatologie | |||||||
Numărul 1(69) / 2016 / ISSN 1810-5289 | |||||||
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CZU: 618.3-007-06-036.17-07-053.1 | |||||||
Pag. 38-43 | |||||||
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Background. There are numerous clinical cases that demonstrate chronic colostasis and megacolon, when intestinal nerve plexus are present, having however defective anatomical and structural histopathological peculiarities. A research at molecular level of immunohistochemical expression of neurotrophic monoclonal antibodies presents an interest. Material and methods. The study material was intestinal tissue samples taken intraoperationally from 117 patients who underwent surgery on congenital intestinal malformations. There have been used the hematoxylin-eosin and picrofucsin staining methods after van Gieson, trichrome Masson and primary monoclonal and polyclonal Neuron Specifi c Enolasae (NSE), Neurofi lament Protein (NFP), Chromogranin A (CGA), Synaptophisyn (SYP) and Smooth Muscle Actin (SMA) antibodies. Results. Classical methods of morphopathologic investigation offers a wide range of possibilities for evaluating structural and functional peculiarities of the colon within colon congenital malformations, including peculiarities of colonic nervous apparatus, as well as defi ning combined colon malformations. However, immunohistochemical researches allow evaluating histopathological particularities at molecular level, making possible the assessment of the resected colon functionality level, especially with regard to the areas visually and intraoperationally healthy. Conclusion. Classical methods have a valuable impact on detecting tissue structures in time and in different variations of congenital structural disturbances, and the use of neurotrophic monoclonal antibodies provides data on neuronal and synaptic structural components, which without doubt promotes the use of these antibodies in differential diagnosis of intestinal transit disorders resulting in megacolon, as well as in assessing the quality of local nervous apparatus at level of resected intestine. |
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Cuvinte-cheie Congenital intestinal malformations, morphopathologic particularities, primary monoclonal antibodies, nerve plexus |
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