Adsorption of bacillus subtilis and bacillus cereus bacteria on enterosorbent obtained from vegetal raw material
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LUPAŞCU, Lucian, PETUHOV, Oleg, TIMBALIUC, Nina, LUPASCU, Tudor. Adsorption of bacillus subtilis and bacillus cereus bacteria on enterosorbent obtained from vegetal raw material. In: Ecological chemistry ensures a healthy environment, 16 septembrie 2022, Chisinau. Chişinău: Institute of Chemistry, 2022, p. 26. DOI: https://doi.org/10.19261/enece.2022.ab17
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Ecological chemistry ensures a healthy environment 2022
Seminarul "Ecological chemistry ensures a healthy environment. "
Chisinau, Moldova, 16 septembrie 2022

Adsorption of bacillus subtilis and bacillus cereus bacteria on enterosorbent obtained from vegetal raw material

DOI:https://doi.org/10.19261/enece.2022.ab17

Pag. 26-26

Lupaşcu Lucian, Petuhov Oleg, Timbaliuc Nina, Lupascu Tudor
 
Institute of Chemistry
 
Proiecte:
 
Disponibil în IBN: 3 octombrie 2022


Rezumat

One of the main problems of modern medicine of this century is intoxication of a human organism as a result of the direct impact of the negative state of the environment on a human. Enterosorption is one of the safest and most effective methods for removing toxic substances, based on oral administration of drugs that can absorb various toxic substances of endogenous and exogenous origin in the lumen of the gastrointestinal tract without entering into chemical reactions with them [1]. Are presented the results of adsorption of 2 species of gram-positive bacteria, such as: Bacillus subtilis, Bacillus cereus on enterosorbent AC-A obtained from vegetal raw material. The mentioned bacterial species were isolated from soil samples and grown on nutrient agar for the accumulation of the biomass. The adsorption of bacteria was studied as a function of temperature (26 and 36oC) and the pH of the solution (1.97-4.05). The equilibrium of the adsorption process of B. subtilis bacteria is established after 75 minutes of stirring, and in the case of B. cereus bacteria the equilibrium is established in 90 minutes. The analysis of the adsorption isotherms of the bacteria allow to conclude that the adsorption process of the bacteria is an exothermic one, i.e. with the increase of the temperature, the adsorption value decreases. The pH value of the solution has a different influence. In the case of B. subtilis, the decrease in pH leads to an increase in the amount of immobilized bacteria per unit area of carbon adsorbent, and in the case of B. cereus bacteria the effect is the opposite, a decrease in pH leads to a decrease in the number of immobilized bacteria on the carbon enterosorbent. Using Scanning Electron Microscopy (SEM), it has been shown that the immobilization of bacteria takes place on the outer surface of the carbon adsorbent. The studied bacteria are adsorbed on the outer surface of the carbonic enterosorbent. This is explained by the fact that the length of the bacteria is within the dimensions of 1-6 μm, a size that exceeds several times the diameter of the macropores of the carbon adsorbent.