Bacteria of the genus Rhodococcus play an important role both in the processes of soil formation and in their use for bioremediation of soils, as well as for wastewater treatment [1]. Along with this, immobilized Rhodococcus cells on various substrates have shown that they can act as effective biocatalysts for the decomposition of carbon-containing pollutants. The interaction of Rhodococcus rhodochrous CNMN-Ac-05 with Fe3O4/PVP nanoparticles was studied earlier and the effect on cells viability and morphological variability was determined [2]. Thus, the combination of magnetic ferrites and bacterial cells is interesting. Fig.1 illustrates the results of incubation of Rhodococcus rhodochrous bacterial cells with magnetic nanoparticles stabilized with polyethylene glycol CoFe2O4/PEG of dimension 80 – 150 nm, collected in glucose medium after 1, 5 and 24 hours. The study was carried out using a confocal laser microscope ZEISS LSM 900 (Carl Ziess, Germany). A B C Fig.1. Microfotographs of the bacteria Rhodococcus rhodochrous CNMN-Ac-05 attached to the surface of Nps CoFe2O4/PEG, at magnification 630 after 1 (A), 5 (B), 24 (C) hours. The method of Confocal laser microscopy allows to visualize directly the localization of bacterial cells on nanoparticles. Over time, we observe sequential adherence of bacterial cells to agglomerated nanoparticles and this process is irreversible, which indicates the predominance of chemical interaction over electrostatic, and this is obviously the result of influence of polyethylene glycol film formed on nanoparticles during synthesis. The observed effect can be used for creation of a nanobiocomposite eliminating of pollutants.