Adsorption of bacillus cereus, bacillus subtilis and pseudomonas fluorescens bacteria from acid water solutions on activated carbon
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LUPAŞCU, Lucian, PETUHOV, Oleg, LUPASCU, Tudor. Adsorption of bacillus cereus, bacillus subtilis and pseudomonas fluorescens bacteria from acid water solutions on activated carbon. In: Ecological and environmental chemistry : - 2022, Ed. 7, 3-4 martie 2022, Chișinău. Chisinau: Centrul Editorial-Poligrafic al USM, 2022, Ediția 7, Vol.1, pp. 97-98. ISBN 978-9975-159-07-4.. 10.19261/eec.2022.v1
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Ecological and environmental chemistry
Ediția 7, Vol.1, 2022
Conferința "Ecological and environmental chemistry 2022"
7, Chișinău, Moldova, 3-4 martie 2022

Adsorption of bacillus cereus, bacillus subtilis and pseudomonas fluorescens bacteria from acid water solutions on activated carbon

CZU: 542.06:[544.7+661.183.2]

Pag. 97-98

Lupaşcu Lucian, Petuhov Oleg, Lupascu Tudor
 
Institute of Chemistry, MSU
 
Proiecte:
 
Disponibil în IBN: 9 martie 2022


Rezumat

Microorganisms can be dangerous contaminants, and their elimination is very important for human and animal health. Adsorption is often used at the end of a treatment sequence due to a high degree of purification that can be achieved. Activated carbon is the most popular adsorbent used for the application of the adsorption technique. They represent complex and heterogeneous materials and can be obtained from different plant sources with unique adsorption characteristics influenced mainly by the porous structure, surface and chemical structure of the surface. Granular activated carbon has an extremely large adsorption surface, which offers an exceptional ability to absorb many types of materials on its surface. There is some evidence that the additional use of activated carbon in the treatment of people with gastroenteritis has shortened the duration of diarrhea and reduced the recovery time compared to standard rehydration monotherapy. Activated carbon has a strong ability to attract and eliminate ingested toxins from the gastrointestinal tract, thus preventing their adsorption. The porous texture of the carbon has a negative electrical charge, which makes it attract positively charged molecules, such as bacterial toxins and intestinal gases. So, preventing the adsorption in the body of these adverse entities, makes them suitable for the treatment of diarrhea. Because the normal stomach pH is in the range of 1-2, this study was performed to test the ability of activated carbon obtained from apricot peels to adsorb bacteria from the gram-positive and gram-negative groups at pH 1.97. To evaluate the bacterial adsorption properties of the activated carbon bacteria from 3 different species (Bacillus cereus, Bacillus subtilis and Pseudomonas fluorescens) were cultivated on solid pepton agar medium. Subsequently, 3 different bacterial solutions were prepared with an initial optical density of 1.5 after McFarland. The same amount of activated carbon obtained from apricot peels was added to the prepared solutions, around 100 mg. The contact time of the carbon and bacteria varied from 30 to 120 min. The experiments were performed at pH 1.97. After each measurement, the adsorption isotherms were established for each selected bacterium. Bacterial species The maximum value of the adsorption of bacteria on carbon adsorbents at pH 1,97 (McF*108/g) B. cereus 30 min 60 min 90 min 120 min 0,095 0,112 0,19 0,26 Ps. Fluorescens 0,075 0,14 0,17 0,20 B.subtilis 0,330 0,340 0,450 0,470 The results in the table show that the adsorption process has a specific character, the strongest adsorption being manifested for B. subtilis bacteria.