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Ultima descărcare din IBN: 2024-01-28 03:10 |
SM ISO690:2012 COBZAC, Vitalie, VERESTIUC, Liliana, JIAN, Mariana, NACU, Viorel. Grefe obținute prin inginerie tisulară în tratamentul defectelor de cartilaj articular. In: Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, 21-23 octombrie 2020, Chişinău. Chişinău: USMF, 2020, p. 500. |
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Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu” 2020 | ||||||
Congresul "Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”" Chişinău, Moldova, 21-23 octombrie 2020 | ||||||
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Background. Articular cartilage lesions are common in patients with injuries of large joints. Cartilage defects can lead to the joint osteoarthritis. Mosaicplasty and tissue engineering techniques are used today in the treatment of such lesions. Objective of the study. The in vivo testing of obtained allogeneic demineralized and decellularized osteochondral graft (DDOG), combined with autologous bone marrow mesenchymal stem cells (MSC) and chondrocytes, for articular cartilage repair. Material and Methods. The rabbit distal femurs were demineralised in 0.6M HCl. Pieces of osteochondral tissue with 3.7 mm in diameter were cutted and decellularized in 1% SDS. The autologous MSC (n=7) and chondrocytes (n=7) were isolated and cultured, then seedet on the graft and transplanted in a knee osteochondral defect. In the control group the rabbits were treated by Mosaicplasty technique (n=7). Results. Cartilage regeneration was assessed by the Unicamp Partial Thickness Score (SGPU) at 12 weeks after transplantation. Practically we did not find a significant difference between the group treated with DDOG combined with MSC and the one treated with DDOG combined with chondrocytes even if the first one statistically shows better results (p˃ 0.5). At the same time, there is a significant difference between the results obtained after the treatment with DDOG combined with MSC compared to the control group (p˂ 0.1) and when comparing the group treated with DDOG combined with chondrocytes with the control group (p˂ 0.5). Conclusion. Utilisation of DDOG combined with MSC and chondrocytes is more effective in regeneration of a critical experimental cartilage defect if compared to Mosaicplasty. DDOG with MSC can be implemented in clinical practice because it is one step surgery and does not bring additional trauma to the joint. |
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Cuvinte-cheie cartilage lesion, mesenchymal stem cells, chondrocytes, leziune cartilaj, celule stem mezenchimale, condrocite, grefă |
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