The purpose of this study is assessment of water use in the Central Region of the Republic of Moldova. The main topics presented in this paper are: 1) assessment of water resources in the Central Region of the Republic of Moldova; 2) tendencies of water consumption on the abstracted sources and on the main usage categories; 3) spatial and branch analysis of water use; 5) the main problems of water use and protection in the region of study. In the region of study are concentrated about 2/3 of groundwater reserves of Republic of Moldova. From surface sources is abstracted ≈3/4 of total volume of water used and over 60% of water is used by households. Outside of Chisinau, over 70% of the water is abstracted from underground sources and is used for various agricultural needs. In the 2007-2017 years, the volume of water use registers a significant decrease which is conditioned, mainly, by decreasing of water abstracted from surface sources and used for agricultural activities, especially for irrigation.