Comparison of two immobile phases used in HPLC analysis of organic acids in wine
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STURZA, Rodica, LAZACOVICI, Olga. Comparison of two immobile phases used in HPLC analysis of organic acids in wine. In: Modern Technologies in the Food Industry, Ed. 1, 1-3 noiembrie 2012, Chişinău. Chișinău, Republica Moldova: 2012, Vol.2, pp. 304-307. ISBN 978-9975-87-428-1.
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Modern Technologies in the Food Industry
Vol.2, 2012
Conferința "Modern Technologies in the Food Industry"
1, Chişinău, Moldova, 1-3 noiembrie 2012

Comparison of two immobile phases used in HPLC analysis of organic acids in wine


Pag. 304-307

Sturza Rodica12, Lazacovici Olga1
 
1 National Center for Quality Testing of the Alcoholic Beverages,
2 Technical University of Moldova
 
Disponibil în IBN: 5 octombrie 2020


Rezumat

Organic acids are one of the most important substances in wine, which make major contributions to the composition, stability and organoleptic qualities of wines, responsible of wines’ microbiological and physicochemical stability. For searching of optimal conditions for HPLC analysis of organic acids in wine there were investigated two immobile phases, such as: modified C18 type and column, in which separation of the analytes is based on ion exclusion and ion exchange effects. Thus NUCLEODUR C18 Pyramid (Macherey-Nagel) is a silica phase with hydrophilic endcapping, designed especially for use in eluent systems of up to 100 % water. The NUCLEOGEL SUGAR 810 H (Macherey-Nagel) is packed with a sulphonated spherical polymer of gel type in the H+ form. It is specifically designed for the separation of numerous polar organic compounds like organic acids, alcohols, sugar alcohols and sugars.

Cuvinte-cheie
HPLC, separation of polar compounds, organic acids, reversed phase