The eggs treatment process is one from which the result of incubation largely depends. In incubation for eggs disinfection are used different methods that affect the bacterial load. Choosing the most effective methods of eggs disinfection will depend on how used substances will influence on embryonic and postembryonic development of poultry. Currently the use of substances having disinfectant effect which would not have negative influence during embryonic and postembryonic development is one of the latest trends. As an alternative to chemical treatment methods it was proposed the use of propolis extract in hens’ egg incubation. The aim of research was to determine the influence of propolis extract on hatching indices used in incubation of hens’ eggs. At the end of the experience and data recording there was established that the maximum index of eggs hatchability was received as a result of using as a disinfectant the propolis extract in an amount of 2 ml/70 ml of water and 4 ml/70 ml water daily during the whole period of incubation. Hatchability was higher in first experimental group compared to the control group and second experimental group.