In the last years a serious attention was given to the implementation of the UNECE Protocol on Water and Health, including the development of a model for the participation of the region's sanitary services in transboundary water monitoring and management. The objective of this component was to create a platform for the effective cooperation of the sanitary services of the basin in accordance with art. 13 of the Protocol: “Where any Parties border the same transboundary waters, …, they shall cooperate and, as appropriate, assist each other to prevent, control and reduce transboundary effects of water-related disease.”. To create a good platform for effective participation, we had to combine the efforts of the sanitary and epidemiological services of the river basin to control the quality of transboundary waters and prevent waterborne diseases. This idea had a success: the model was launched, it was initiated the activity of the joint Moldovan-Ukrainian working group on sanitaryhygienic monitoring of transboundary waters. As a result, within the framework of the project, the working group developed a draft “Regulations for cooperation on sanitary and hygienic monitoring of water quality in the transboundary Moldovan-Ukrainian sections of the Dniester River basin”, designed to become the official mechanism of interaction between the sanitary services of the basin. The implementation of the provisions of such Regulations in the future can provide a good opportunity to work together to improve monitoring, as well as to prevent the negative impact of water quality on human health. A representative of a public environmental organization became the coordinator of such a working group by mutual consent of the countries. It speaks about the democratic principles of the approach to solving the set tasks.Sanitary doctors from different areas of the basin: Chisinau, Odessa region, Vinnitsa, Cernauţi, and Transdniestria (Tiraspol) were involved in the work of the working group, which indicates compliance with the principles of an integrated approach to the development of common and effective documents and models of collaboration. Such a model of effective participation of sanitary-epidemiological services passed a good practice of cooperation and implementation. The draft Regulation was based on Art. 6 of the 1994 Inter-government Agreement on Use of Boundary Waters between Moldova and Ukraine. The purpose of the Regulation was to determine the main criteria for the sanitary-hygienic assessment of the quality of transboundary waters in the Dniester River Basin in order to obtain comparable measurement data on water quality indicators, the basis of which it is possible to jointly assess the quality of transboundary waters. The elaborated cooperation model permitted to demonstrate the effectiveness of such cooperation, especially to prevent the waterborne human diseases and to determine the sources of infections. It is important for now to include these activities in everyday interstate water cooperation of Moldova and Ukraine in frames of the Dniester River Commission working groups and governmental agencies responsible for human health and environment.