Hyssopus officinalis (L) is one of the most frequently consumed herbal remedies as a minty flavour, condiment and spices in food industries as well. Nowadays, the therapeutic uses and health benefits of hyssop are most based on ethno pharmacy and folklore rather than on scientific argumentation. Despite the fact that the wide range of information suggesting health benefits of herbal remedies associated with hyssop, evidence-based information regarding the effects of hyssop is quite limited. Turning to account an active herbal cure is based to a better understanding of the relationship between chemical constituents and biological properties of the natural product. In view of these aspects, natural products, particularly higher plant species, continue to be important sources of medicine and supplementary health products which represent a challenge to science due to their various properties, including chemical diversity, synergism to biological activity and variable compositions. Herein, we tried to conducting research expected morphological, chemical and genetic hyssop different varieties. Identification and characterization of chemotypes was made in the study of the composition of essential oils by GC, HPLC etc. It will identify genetic variability based on RAPD profiles. It proposes a number of issues on inheritance morphological and biochemical characters. It will estimate the association between morphological, biochemical, and genetic and environmental effects on the formation of valuable quantitative characters. Concisely, these issues furnish the background for the experiments on the associated basic studies for the H. officinalis (L.) which is an important source of bioactive substances of medicinal interest. The results allow the characterization of the studied species genetic resources that can be used in biodiversity conservation strategies and detection of PMA forms for the inclusion of improvement. Based on these studies will assess the possibility of developing molecular markers and their association with quantitative morphological and biochemical characters. Markers give will be used to identify sources and facilitating valuable new genetic marker assisted improvement (ASMM).