|Ultima descărcare din IBN:|
| SM ISO690:2012|
TISHKOV, Vladimir. Enzymes as Nanobiocatalysts: Structural Studies and Engineering. In: NANO-2019: Limits of Nanoscience and Nanotechnologies. 24-27 septembrie 2019, Chişinău. Chișinău, Republica Moldova: 2019, p. 13.
|NANO-2019: Limits of Nanoscience and Nanotechnologies 2019|
Conferința "SPINTECH Summer school “S/F Hybrid Structures for Spintronics”" |
2019, Chişinău, Moldova, 24-27 septembrie 2019
Enzymes are nanosized biocatalysts with very high efficiency and specificity. At present biocatalytic processes are introduced in many fields of industry, agriculture and analysis. Unfortunately, practical application of enzyme is limited due to low activity with unnatural compounds. The other drawbacks are high cost of production, insufficient thermal and operational stability. In our laboratory we carry out genetic engineering and protein design experiments to prepare biocatalysts for processes of chiral synthesis, β-lactam antibiotics preparation, biosensors for environmental analysis and medical diagnostics as well as for construction of new drugs. Cost of production is decreased by construction of recombinant strains which overproduce enzymes of interest. Experiments for improving thermal and operational stability are necessary to reduce production and storage costs as well as to increase enzyme working time in process itself. In our study we use “rational design” approach which requires knowledge of 3-dimentional structure of enzyme. Different formate dehydrogenases – from bacterium Staphylococcus aureus, yeast Ogataea parapolymorpha DL-1 and moss Physcomitrella patens, apo- and holo-forms were crystallized and 3D X-ray structures were determined. Protein engineering experiments resulted in preparation of biocatalysts with improved and new properties.