The effect of controlled reperfusion on experimental ovarian torsion
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LAZĂR, Cornelia; VOZIAN, Marin; PANTEA, Valeriana; ŞVEŢ, Inna; MISHINA, Ana; TAGADIUC, Olga. The effect of controlled reperfusion on experimental ovarian torsion. In: Russian Open Medical Journal. 2019, nr. 4(8), p. 0. ISSN 2304-3415.
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Russian Open Medical Journal
Numărul 4(8) / 2019 / ISSN 2304-3415

The effect of controlled reperfusion on experimental ovarian torsion


DOI: 10.15275/rusomj.2019.0404
Pag. 0-0

Lazăr Cornelia1, Vozian Marin1, Pantea Valeriana1, Şveţ Inna1, Mishina Ana2, Tagadiuc Olga1
 
1 ”Nicolae Testemițanu” State University of Medicine and Pharmacy,
2 Mother and Child Institute
 
Disponibil în IBN: 16 ianuarie 2020


Rezumat

The objective of our research was to study the effect of controlled reperfusion on lactate and advanced oxidation protein products (AOPP) in experimental ovarian torsion (OT), and to evaluate the possibility to reduce the reperfusion injuries applying the ON-OFF technique of reperfusion. Material and Methods - Lactate and AOPP were measured in serum and ovarian homogenates of 70 females rats, divided equally into 7 groups: no intervention (nr. 1); laparotomy (nr. 2); 3 hours OT (nr. 3); 3 hours OT + 1 hour (nr. 4) or 24 hours (nr. 6) of simple reperfusion (SR); 3 hours OT + 1 hour (nr. 5) or 24 hours (nr. 7) of controlled reperfusion (ON-OFF). The results were analyzed using Welch's ANOVA. Results - OT produced an increase of serum (66%, p<0.001) and homogenate (38%, p=0.006) lactate, that tended to return to baseline in 24 hours despite the type of reperfusion. AOPP had grown in OT animals serum (20%, p=0.007) and homogenate (26%, p>0.05) and continued to rise in the serum after SR, while in ON-OFF technique groups - decreased by about 20% (p<0.01). Homogenate AOPP levels rised after reperfusion in all groups. Conclusion - Lactate is a biomarker for ischemia in OT. Increased AOPP in experimental groups indicates that there is a degree of oxidative stress that can affect the cells after the restoration of the blood flow. Therefore, the correct management of the condition that reduces reactive oxygen species production, such as controlled method of reperfusion, could be appropriate to prevent additional injuries.

Cuvinte-cheie
Advanced oxidation protein products, Controlled reperfusion, Lactate, Ovarian torsion, Reperfusion injury