Metodă nouă de apreciere a toxicităţii substanţelor chimice
Închide
Articolul precedent
Articolul urmator
699 11
Ultima descărcare din IBN:
2023-10-23 10:58
Căutarea după subiecte
similare conform CZU
[543:541.6]:615.9 (1)
Chimie analitică (319)
Toxicologie generală. Studii generale asupra otrăvurilor şi otrăvirii (intoxicaţiei) (45)
SM ISO690:2012
TODERAŞ, Ion, GULEA, Aurelian, GUDUMAC, Valentin, ROSCOV-ARCAN, Elena, GARBUZ, Olga. Metodă nouă de apreciere a toxicităţii substanţelor chimice. In: Functional Ecology of Animals: 70th anniversary from the birth of academician I.Toderas, 21 septembrie 2018, Chişinău. Chișinău, Republica Moldova: Institutul de Zoologie, 2018, pp. 454-463. ISBN 978-9975-3159-7-5. DOI: https://doi.org/10.53937/9789975315975.81
EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core
Functional Ecology of Animals 2018
Conferința "Functional Ecology of Animals"
Chişinău, Moldova, 21 septembrie 2018

Metodă nouă de apreciere a toxicităţii substanţelor chimice

DOI:https://doi.org/10.53937/9789975315975.81
CZU: [543:541.6]:615.9

Pag. 454-463

Toderaş Ion1, Gulea Aurelian2, Gudumac Valentin3, Roscov-Arcan Elena2, Garbuz Olga2
 
1 Institutul de Zoologie,
2 Universitatea de Stat din Moldova,
3 Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu“
 
 
Disponibil în IBN: 26 noiembrie 2019


Rezumat

We developed a new invention that relates to method for assessing the toxicity of chemical substances.The method of the invention includes the preparation of the culture of Paramecium caudatum, adding to the samples investigated to test chemicals in various concentrations, incubating the sample to be studied and control the addition of colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, incubation with subsequent addition of solution of formalin, centrifuging the sample, removing the supernatant, adding the solution of hydroxide of sodium, determine the absorbance using a spectrophotometer, followed by calculating the percentage of paramecii viability and determine the lethal concentration (LC50) at the same time as the value of the concentration LC50 is small, the toxicity of the tested substance is higher.

Cuvinte-cheie
Paramecium caudatum, chemical substances, colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, lethal concentration (LC50), spectrophotometer