Articolul precedent |
Articolul urmator |
699 11 |
Ultima descărcare din IBN: 2023-10-23 10:58 |
Căutarea după subiecte similare conform CZU |
[543:541.6]:615.9 (1) |
Chimie analitică (319) |
Toxicologie generală. Studii generale asupra otrăvurilor şi otrăvirii (intoxicaţiei) (45) |
SM ISO690:2012 TODERAŞ, Ion, GULEA, Aurelian, GUDUMAC, Valentin, ROSCOV-ARCAN, Elena, GARBUZ, Olga. Metodă nouă de apreciere a toxicităţii substanţelor chimice. In: Functional Ecology of Animals: 70th anniversary from the birth of academician I.Toderas, 21 septembrie 2018, Chişinău. Chișinău, Republica Moldova: Institutul de Zoologie, 2018, pp. 454-463. ISBN 978-9975-3159-7-5. DOI: https://doi.org/10.53937/9789975315975.81 |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Functional Ecology of Animals 2018 | ||||||
Conferința "Functional Ecology of Animals" Chişinău, Moldova, 21 septembrie 2018 | ||||||
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DOI:https://doi.org/10.53937/9789975315975.81 | ||||||
CZU: [543:541.6]:615.9 | ||||||
Pag. 454-463 | ||||||
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Rezumat | ||||||
We developed a new invention that relates to method for assessing the toxicity of chemical substances.The method of the invention includes the preparation of the culture of Paramecium caudatum, adding to the samples investigated to test chemicals in various concentrations, incubating the sample to be studied and control the addition of colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, incubation with subsequent addition of solution of formalin, centrifuging the sample, removing the supernatant, adding the solution of hydroxide of sodium, determine the absorbance using a spectrophotometer, followed by calculating the percentage of paramecii viability and determine the lethal concentration (LC50) at the same time as the value of the concentration LC50 is small, the toxicity of the tested substance is higher. |
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Cuvinte-cheie Paramecium caudatum, chemical substances, colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, lethal concentration (LC50), spectrophotometer |
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