Phaseolin subjected to limited proteolysis by papain-like proteinase: Primary structure and crystallization

Rotari Vitalie; Prak Krisna; Stepurina Tatiana; Morari Diana; Lapteva Natalya; Mikami Bunzo; Utsumi Shigeru; Wilson Karl; Shutov Andrei

Conţinutul numărului revistei

Articolul precedent

Articolul urmator

989

Ultima descărcare din IBN:
2022-07-09 11:42

SM ISO690:2012

ROTARI, Vitalie, PRAK, Krisna, STEPURINA, Tatiana, MORARI, Diana, LAPTEVA, Natalya, MIKAMI, Bunzo, UTSUMI, Shigeru, WILSON, Karl, SHUTOV, Andrei. Phaseolin subjected to limited proteolysis by papain-like proteinase: Primary structure and crystallization. In: Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii), 2008, nr. 2(12), pp. 19-22. ISSN 1814-3237.

EXPORT metadate:
Google Scholar
Crossref
CERIF

DataCite
Dublin Core

Studia Universitatis Moldaviae (Seria Ştiinţe Reale şi ale Naturii)

Numărul 2(12) / 2008 / ISSN 1814-3237 /ISSNe 1857-498X

Phaseolin subjected to limited proteolysis by papain-like proteinase: Primary structure and crystallization

Pag. 19-22

Rotari Vitalie, Prak Krisna, Stepurina Tatiana, Morari Diana, Lapteva Natalya, Mikami Bunzo, Utsumi Shigeru, Wilson Karl, Shutov Andrei

Moldova State University

Disponibil în IBN: 11 decembrie 2013

Descarcă PDF

Rezumat

Fazeolina, globulina de rezervă 7S din cotiledoanele seminţelor de Phaseolus vulgaris a fost supusă proteolizei limitate cu proteinaza papainică endogenă PP purificată din cotiledoanele plantulelor de fasole de 6 zile. Digestul a fost separat în două fracţii: prima cu masă moleculară mare (fazeolina-PP care reţine structura cuaternară), iar a doua cu masă moleculară mică (peptide eliberate). Structura primară completă a fazeolinei-PP a fost descrisă în baza rezultatelor combinate a mass-spectrometriei peptidelor eliberate şi a secvenării amino-terminale a fazeolinei-PP. Au fost obţinute câteva tipuri de cristale ale fazeolinei-PP. Cel mai bun cristal cu rezoluţia difracţiei razelor X de 5,5 Å a fost găsit că aparţine grupei spaţiale P6322, care diferă de grupa spaţială a cristalelor fazeolinei intacte. Este discutat rolul posibil al proteolizei limitate a fazeolinei induse de PP la degradarea ei in vivo.

Cerif XML Export

<?xml version='1.0' encoding='utf-8'?>
<CERIF xmlns='urn:xmlns:org:eurocris:cerif-1.5-1' xsi:schemaLocation='urn:xmlns:org:eurocris:cerif-1.5-1 http://www.eurocris.org/Uploads/Web%20pages/CERIF-1.5/CERIF_1.5_1.xsd' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' release='1.5' date='2012-10-07' sourceDatabase='Output Profile'>
<cfResPubl>
<cfResPublId>ibn-ResPubl-8802</cfResPublId>
<cfResPublDate>2008-02-01</cfResPublDate>
<cfVol>12</cfVol>
<cfIssue>2</cfIssue>
<cfStartPage>19</cfStartPage>
<cfISSN>1814-3237</cfISSN>
<cfURI>https://ibn.idsi.md/ro/vizualizare_articol/8802</cfURI>
<cfTitle cfLangCode='EN' cfTrans='o'>Phaseolin subjected to limited proteolysis by papain-like proteinase: Primary structure and crystallization</cfTitle>
<cfAbstr cfLangCode='RO' cfTrans='o'>Fazeolina, globulina de rezervă 7S din cotiledoanele seminţelor de Phaseolus vulgaris a fost supusă proteolizei
limitate cu proteinaza papainică endogenă PP purificată din cotiledoanele plantulelor de fasole de 6 zile. Digestul a fost
separat în două fracţii: prima cu masă moleculară mare (fazeolina-PP care reţine structura cuaternară), iar a doua cu
masă moleculară mică (peptide eliberate). Structura primară completă a fazeolinei-PP a fost descrisă în baza rezultatelor
combinate a mass-spectrometriei peptidelor eliberate şi a secvenării amino-terminale a fazeolinei-PP. Au fost obţinute
câteva tipuri de cristale ale fazeolinei-PP. Cel mai bun cristal cu rezoluţia difracţiei razelor X de 5,5 Å a fost găsit că
aparţine grupei spaţiale P6322, care diferă de grupa spaţială a cristalelor fazeolinei intacte. Este discutat rolul posibil al
proteolizei limitate a fazeolinei induse de PP la degradarea ei in vivo.</cfAbstr>
<cfResPubl_Class>
<cfClassId>eda2d9e9-34c5-11e1-b86c-0800200c9a66</cfClassId>
<cfClassSchemeId>759af938-34ae-11e1-b86c-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfResPubl_Class>
<cfResPubl_Class>
<cfClassId>e601872f-4b7e-4d88-929f-7df027b226c9</cfClassId>
<cfClassSchemeId>40e90e2f-446d-460a-98e5-5dce57550c48</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfResPubl_Class>
<cfPers_ResPubl>
<cfPersId>ibn-person-1319</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-15346</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-12555</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-1281</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-1258</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-33212</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-33185</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-33183</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
<cfPers_ResPubl>
<cfPersId>ibn-person-10615</cfPersId>
<cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId>
<cfClassSchemeId>b7135ad0-1d00-11e1-8bc2-0800200c9a66</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
</cfPers_ResPubl>
</cfResPubl>
<cfPers>
<cfPersId>ibn-Pers-1319</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-1319-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Ротарь</cfFamilyNames>
<cfFirstNames>Виталие</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-15346</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-15346-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Prak</cfFamilyNames>
<cfFirstNames>Krisna</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-12555</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-12555-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Степурина</cfFamilyNames>
<cfFirstNames>Т.</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-1281</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-1281-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Morari</cfFamilyNames>
<cfFirstNames>Diana</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-1258</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-1258-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Lapteva</cfFamilyNames>
<cfFirstNames>Natalya</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-33212</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-33212-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Mikami</cfFamilyNames>
<cfFirstNames>Bunzo</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-33185</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-33185-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Utsumi</cfFamilyNames>
<cfFirstNames>Shigeru</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-33183</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-33183-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Wilson</cfFamilyNames>
<cfFirstNames>Karl</cfFirstNames>
</cfPersName_Pers>
</cfPers>
<cfPers>
<cfPersId>ibn-Pers-10615</cfPersId>
<cfPersName_Pers>
<cfPersNameId>ibn-PersName-10615-3</cfPersNameId>
<cfClassId>55f90543-d631-42eb-8d47-d8d9266cbb26</cfClassId>
<cfClassSchemeId>7375609d-cfa6-45ce-a803-75de69abe21f</cfClassSchemeId>
<cfStartDate>2008-02-01T24:00:00</cfStartDate>
<cfFamilyNames>Shutov</cfFamilyNames>
<cfFirstNames>Andrei </cfFirstNames>
<cfFamilyNames>Шутов</cfFamilyNames>
<cfFirstNames>Андрей</cfFirstNames>
</cfPersName_Pers>
</cfPers>
</CERIF>