Kinetics model of peroxidation process in nanocomposite biosystem such as mitochondrial membrane of cell
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YALTYCHENKO, Olga, KANAROVSKY, Evghenii, GORINCHOY, Natalia. Kinetics model of peroxidation process in nanocomposite biosystem such as mitochondrial membrane of cell. In: Achievements and perspectives of modern chemistry, 9-11 octombrie 2019, Chişinău. Chisinau, Republic of Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2019, p. 247. ISBN 978-9975-62-428-2.
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Achievements and perspectives of modern chemistry 2019
Conferința "International Conference "Achievements and perspectives of modern chemistry""
Chişinău, Moldova, 9-11 octombrie 2019

Kinetics model of peroxidation process in nanocomposite biosystem such as mitochondrial membrane of cell


Pag. 247-247

Yaltychenko Olga1, Kanarovsky Evghenii1, Gorinchoy Natalia2
 
1 Institute of Applied Physics,
2 Institute of Chemistry
 
Disponibil în IBN: 12 noiembrie 2019


Rezumat

Currently, the widespread use of the physical measurement methods in all domains of bioscience has greatly enhanced the capabilities of biochemical methods of analysis and has caused the intensive growth of biotechnology. Thus, the large-scale studies of processes and phenomena in the natural composite biosystems, stimulate not only the study of their properties and structural features, but also the elaboration and creation of various artificial biocomposite materials with the desired properties, which is extremely important for the development of medicine and pharmacology. One of the most extensive classes of the natural composite biosystems are the cell membranes (CM) of animals and plants, as well as their artificial analogs based on the lipid mono- and bilayers, having a flat or spherical shape. According to this model, the CM on its surface and inside its structure contains a mosaic of disordered inclusions of various protein molecules (performing the transport, signaling, channel-forming and catalytic functions). In addition, the lipid bilayer, which consists of various saturated and unsaturated lipids, being the supporting base of the CM, is also a disordered mosaic system of nanoscale fragments that have a liquid crystalline structure and vary in size and composition. Thus, a biosystem of this kind can be considered as an amorphous compound representing a chaotically coupled set of the various nanoscale fragments. The given work is devoted to the modeling of the peroxidase activity of the cytochrome c (Cyt) and cardiolipin (CL) complexes, due to which the process of lipid peroxidation (LPO) occurs in the mitochondrial membranes (MMs). The formation of the Cyt-CL complexes that manifest a quite high peroxidase activity, both in vivo (in the MMs) and in vitro (in the artificial analogs of CMs based on the lipid mono- and bi-layers) has been proven experimentally (details on the composition, the structure and causes of the peroxidase activity of Cyt-CL complexes are presented in the review [1]). Also in this work, the influence of lipid antioxidants(by the example of  a-tocopherol and its homologs) on the kinetics of LPO was taken into account. The system of the differential equations relative to the concentrations of reagents was composed following the analysis of the general model reaction scheme consisting of 22 reactions [1] that go on two reaction pathways: enzymatic and non-enzymatic. By performing the numerical simulation and comparing of the yields of the lipid oxidation products, the obtained system of equations allows to determine and compare the effectiveness of the action of various lipid antioxidants on the LPO process; to find the unknown rate constants for some reactions, using the existing experimental data; to evaluate the peroxidase activity of the Cyt-CL complexes including the different types of cardiolipin.