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CĂLUGĂRU-SPĂTARU, Tatiana; CIOCARLAN, Alexandru; DASCALIUC, Alexandru. Comparative study of the productivity of callus biomass and cell aggregates of R. Rosea in vitro. In: Achievements and perspectives of modern chemistry. 9-11 octombrie 2019, Chişinău. Chisinau, Republic of Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2019, p. 210. ISBN 978-9975-62-428-2.
|Achievements and perspectives of modern chemistry 2019|
Conferința "International Conference "Achievements and perspectives of modern chemistry"" |
Chişinău, Moldova, 9-11 octombrie 2019
Golden root (Rhodiola rosea L.) is a well known species of plants, which has been used medicinally for decades, but studies of its pharmacological effects and of the compounds responsible for it use still continue . Unfortunately, lately due to the intensive collection, the distribution area of this species has been substantially reduced. The attempts of industrial cultivation of the species did not produce the desired results. Cell culture of R. rosea represents an alternative method and a model for investigation of the influence at the biological level of organization on the ability of plants to accumulate secondary metabolites. In addition, this method offers the possibility to study the influence of physical and chemical factors under controlled conditions on the accumulation processes. There is information in the literature demonstrating that in some cases the accumulation of secondary metabolites in an in vitro culture is more efficient in a liquid medium (no agar) compared to a solid culture medium (containing agar). Considering this, our studies have been conducted on the influence of these factors on both callus cultures and on aggregates of R. rosea cells grown in a liquid medium . This paper, reports the results of the extraction of the active components from callus and cell aggregates of R. rosea, which represent the amount of substances extracted with different solvents from cell culture cultivated in solid and liquid medium (Table). Table.The results of extractions from callus biomass and cell aggregates of R. roseawith hexane and methanol at the end of the cultivation period (on day 40 and 20, respectively).tabelThe total content of substances extracted from the mass of cell aggregates obtained in the liquid medium is approximately five times higher thanin the biomass of callus.At the same time, the content of extracts with lipophilic properties (HE) is higher in callus cells. Thus, the content of substances of phenolic origin (ME) is significantly higher in cell aggregates grown in a liquid medium. Given that the active principles of R. rosea are mainly represented by phenolic compounds, it becomes clear the priority of cultivating cell aggregates in the liquid medium.