The synthesis of silver nanoparticles by methane-oxidizing bacteria Methylococcus Capsulatus (M)
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2020-06-15 22:04
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AVDEEVA, Lidiya. The synthesis of silver nanoparticles by methane-oxidizing bacteria Methylococcus Capsulatus (M). In: Achievements and perspectives of modern chemistry, 9-11 octombrie 2019, Chişinău. Chisinau, Republic of Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2019, p. 158. ISBN 978-9975-62-428-2.
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Achievements and perspectives of modern chemistry 2019
Conferința "International Conference "Achievements and perspectives of modern chemistry""
Chişinău, Moldova, 9-11 octombrie 2019

The synthesis of silver nanoparticles by methane-oxidizing bacteria Methylococcus Capsulatus (M)


Pag. 158-158

Avdeeva Lidiya
 
Institute of Problems of Chemical Physics, Russian Academy of Sciences
 
Disponibil în IBN: 7 noiembrie 2019


Rezumat

Silver nanoparticles are widely used to obtain various materials with antiseptic properties. According to the principle of impact, methods for producing nanoparticles can be divided into two large groups: dispersion methods for obtaining nanoparticles by grinding a conventional macro sample (chemical, photochemical and radiation reduction, laser evaporation, etc.) and condensation methods for producing nanoparticles from individual atoms (gas condensation, plasma-chemical methods, etc.). By now different microorganisms such as bacterial strains, have been used to develop non-toxic methods to synthesize nanoparticles. Methods of microbial synthesis of nanoparticles are relatively cheap, environmentally safe and extremely diverse. The posibility of synthesis of silver nanoparticles by using methane-oxidizing bacteria Methylococcus capsulatus (M), which iscapable of utilizing methane as their only carbon and energy source was studied in this work. Methylococcus capsulatus (M) cells were cultivated under continuous perfusion conditions in the Ankum 2M fermenter in a standard mineral medium at 42ºC, pH = 5.6. The biomass of microorganisms was separated from the nutrient medium by centrifugation for 10 minutes at 7000 rpm and transferred to solutions of silver nitrate (AgNO3) of various concentrations containing 5 ÷ 1000 mg/L Ag+. In parallel experiments, silver salt solutions were introduced directly into the culture liquid with biomass. Incubation took place at room temperature for up to five days. Extracellular bacterial formation of silver nanoparticles was observed, which was recorded spectrophotometrically. Microparticles with a diameter of one to several microns and larger, rapidly settling particles are also formed with nano-silver. Thus, methane-oxidizing bacteria Methylococcus capsulatus (M) issuitable to use for synthesis of silver nanoparticles