It is known from the literature that copper(II) complexes with salicylaldehyde 4-allylthiosemicarbazone exhibit high antimicrobial activity and the introduction of substituents into the salicylidene fragment leads to a change in antimicrobial activity. The aim of this work is the synthesis of copper coordination compounds of 2,4-dihydroxybenzaldehyde 4-allylthiosemicarbazone (H2L) with 1,10-phenanthroline (1,10-Phen) and 2,2'-bipyridyl (2,2’-Bpy), determination of their composition, structure, physicochemical and biological properties. The experiments showed that, ethanolic solution of copper(II) acetate reacts with 4-allylthiosemicarbazone H2L and 1,10-phenanthroline or 2,2'-bipyridyl forming fine-crystalline colored coordination compounds. The composition of these compounds was determined using elemental analysis for C, H, N, Cu: [Cu(1,10-Phen)(L)], [Cu(2,2’-Bpy)(L)].Single-crystals of these two coordination compounds were obtained as a result of recrystallization from dimethylformamide.Figure. Structure of complexes [Cu(1,10-Phen)(L)]·DMF·H2O and [Cu(2,2’-Bpy)(L)]·DMF. The thiosemicarbazone H2L behaves as double deprotonated tridentate ligand with O,N,Sset of donor atoms and coordinates to the central atom of copper(II) forming five- and sixmembered metallocycles. The fourth and fifth coordination position are occupied by nitrogen atoms of 2,2’-Bpy or 1,10-Phen. These compounds have a monomeric structure (Figure). Synthesized coordination compounds exhibit antimicrobial activity against gram-positive microorganisms Staphylococcus aureus in the range 0.49-1.95 μg/mL and antifungal activity in the range 31.25-500 μg/mL.