Human sexuality covers a large spectrum of attitudes and behavioral patterns. This concept refers to the way people experience and express themselves sexually. For many years, research in the field of human sexuality has been hampered by the lack of attitude assessment instruments suitable for adolescents, university students, and adults. Since the 1980s, several tools have been developed and validated to measure attitudes toward sexuality. One is the ATSS (Attitudes Toward - 22 Sexuality Scale; Fisher & Hall, 1988). This self-report measure has been developed to compare the attitudes toward sexuality of early, middle, and late adolescents and their parents. The ATSS consists of 14 statements dealing with such issues as nudity, abortion, contraception, premarital sex, pornography, prostitution, homosexuality, and sexually transmitted diseases. The instrument has been initially validated on a sample of 141 adolescents between the ages of 12 and 20 and their parents. Fisher and Hall (1988) have reported a fairly consistent body of data on construct validity and reliability of the ATSS. The current study aims to validate the Romanian version of the ATSS using a sample of university and postgraduate students. The participants were 267 young people and adults (218 females and 49 males) with a mean age of 24.2 years. Using confirmatory factor analysis three measurement models were examined and compared with each other. The model with two correlated latent factors and ten items provided the most satisfactory fit to the data. The two factors refer to the liberal/conservative attitude toward sexuality. The Cronbach alpha reliability coefficient for the liberal attitude toward sexuality was .62, and for the conservative attitude is was .81. The construct validity of ATSS was assessed by correlating its subscales with the religiosity and openness to experience. Social desirability response bias, as measured with the Impression Management (IM) subscale from the Paulhus Deception Scale, was found to be weakly associated with ATSS scores. There were statistically significant differences depending on the general orientation on the axis of conservatism-liberalism. The two subscales that we identified for the Romanian version of ATSS can be used alternatively to investigate the attitude toward sexuality among young people.