Complexes with imidazole derivatives have received a great attention because of their interesting spectral and magnetic properties as well as biological activity. Among these, imidazole plays important role in numerous natural compounds and acts as ligand toward transition metal ions in a variety of biologically important systems like iron-heme systems, vitamin B12 and its derivatives, and other several metalloproteins. Taking into account all these, we report here the synthesis and characterization of new complexes of copper and nickel with imidazole derivatives and acrylate ion as ligands. The compounds were characterized by chemical analysis as well as IR, UV-Vis-NIR, EPR spectroscopy and magnetic data at room temperature. All complexes exhibit specific anti-infective properties against Gram-positive (S. aureus, B. subtilis, B. cereus), Gram-negative (P. aeruginosa, E. coli, Enterobacter) and fungal (C. albicans) as demonstrated by the low MIC values. Their ability to inhibit the microbial biofilm formation on inert substratum was also assayed. The thermal analysis (TG, DTA) elucidated the complexes composition as well as the number and the nature of water molecules. The thermal transformations are complex processes according to TG and DTG curves including dehydration, acrylate ion oxidative degradation and thermolysis process of imidazole derivatives. The final product of decomposition is the corresponding metallic oxide.