A glass ceramic material based on CaO- P2O5-Al2O3-SiO2-B2O3-Li2O-Na2O-MgO-ZrO2 was developed to study its bioactive behaviour. Cano and P2O5 were incorporated in the form of natural animal bone ash. ZrO2 added to glasses composition as a nucleation agent with the amounts of 1, 3, 5 % (wt.). Animal bone ash which is a waste product of slaughter houses is commercially used in bone china ceramics. Animal bone ash contains mostly CaO and P2O5. Therefore, the rich content of phosphorous and calcium oxides makes the natural bone ash a potential low-cost raw material to produce bioactive glasses and glass-ceramics [1]. Glass batch based on CaO-P2O5-Al2O3-SiO2-B2O3-Li2O-Na2O-MgO-ZrO2 was successfully melted in alumina crucibles at 1360°C, and frit was obtained by quenching through water at room temperature. The frits were milled for 24 hours to obtain fine glass powders, then pellets were produced from powdered glass by uniaxial pressing and heat treated between 650900°C for two hours. The effect of adding nucleation agent on glass ceramic formation was characterized by DTA, optical microscope and heating microscope. The phases developed in microstructure were identified by XRD and SEM studies. Based on the result obtained from crystallization and microstructural characterization studies the glass-ceramics being heat treated at 800°C for different period of times were chosen for mechanical characterization which were carried out by measuring bulk density, elasticity modulus and flexural strength. The glass ceramic samples, containing 3% ZrO2, that were being heat threated at 800°C for 30 minutes were tested in Simulated Body Fluid (SBF) solution for 3, 7, 14, 21 days. Following invitro test the sample surfaces were analysed by XRD and SEM to detect the nature of bioactivity of glass-ceramics. The other set of samples were used as sintered, and soaked in static condition in order to evaluate the concentration of the elements released by the glass-ceramics. After extraction, SBF was chemically analysed by ICP-OES. The results of in-vitro studies demonstrated that the glass ceramics developed from natural animal bone ash containing glass can be effectively used for the development of bioactive glass ceramics.