Sunflower is one of the most important model crop plants whose commercial hybrid seed production has been obtained using a variety of types of male-sterile lines. Characterization of genes associate with male sterility aims to understand how and why the male sterility occurs, and which proteins are the key players for microspores abortion. Some of genes implicated in this process are represented by GSL12, required for callose biosynthesis that play an important role in deposition of this carbohydrate around pollen mother cell for normal development of microspores and EXPA1, which encode enzyme involved in cell wall extension and cell divisions. To extend our understanding related to expansin and callose functions in sunflower microsporogenesis, it was estimated EXPA1 and GSL12 gene expression in fertile, cytoplasmic (CMS) and induced by gibberellin (IMS) male sterility lines in different meiosis stages. Quantitative real-time PCR was performed using the Maxima SYBR Green/ROX qPCR Master Mix kit according to the manufacturer’s instructions (thermal cycler ДТ-96, DNA technology, Rusia). ΔΔCt method for calculate expression level were used. The results showed the similar pattern of EXPA1 gene expression of both sterile phenotypes. Thus in pachytene, tetrads and microspore stage has been unregistered overexpression and in division – subexpression, comparative with fertile plants. The transcriptional activity of the GSL12 gene was different, depending on the type of sterility. In CMS plants was ascertained overexpression in all microsporogenesis phases, but in IMS plants at pachytene, divisions and tetrads – subexpression with higher value of fold change (-14.1, p<0.05) in the first meiosis stage. Taken together, our data suggest that from the total of the analyzed variants (two genes and 4 phases of microsporogenesis), in CMS plants 87.5% of the cases manifested overexpression and statistically non-significant were 12.5%, in IMS plants 37.5% was overexpression, 50% - subexpression and 12.5 - statistically insignificant (p<0.05).