Storage potential of wheat collection accessions under the conditions of ex situ conservation
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KORLATEANU, L., MIHĂILĂ, Victoria, GANEA, Anatolie. Storage potential of wheat collection accessions under the conditions of ex situ conservation. In: International congress on oil and protein crops, 20-24 mai 2018, Chişinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2018, p. 28. ISBN 978-9975-3178-5-6.
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International congress on oil and protein crops 2018
Congresul "International congress on oil and protein crops"
Chişinău, Moldova, 20-24 mai 2018

Storage potential of wheat collection accessions under the conditions of ex situ conservation


Pag. 28-28

Korlateanu L., Mihăilă Victoria, Ganea Anatolie
 
Institute of Genetics, Physiology and Plant Protection
 
Disponibil în IBN: 31 august 2019


Rezumat

Principal condition of the ensured ex situ conservation of the germplasm in plant gene banks includes the need to maintain seed viability and physiological quality, and to minimize their aging rate. In order to guarantee high storage potential (SP) of seeds, it is essential to create the correct storage conditions for maintenance of the initial germinability. For determination of SP of seeds, gene banks use the accelerated aging test (AA) of seeds involving incubation of seeds under the increased temperature and humidity. With this test it is possible to identify accessions unsuitable for long-term storage, and also to give recommendations with regard to the terms of reproduction of the seed material. Purpose of these investigations was to study morphophysiological and biochemical parameters of wheat seeds and seedlings (Triticum aestivum L.) after the conduction of AA-test. After performance of AA of the soft wheat seeds, the reduction of values of morphophysiological parameters was observed as compared to the control. By the level of decrease of seed germinability, the genotypes split up into 3 groups. The 1st group of genotypes: Moldova 5; Moldova 5 improved; Moldova 5 x Moldova 76 (germinability decreased by 23.0-33.0%); the 2nd group: Moldova 77; Dumbravitsa; Avantaj (germinability decreased by 44.0-47.0%); the 3rd group: Moldova 11; Plai; Talisman (germinability decreased by 55.0-70.0%). The decrease of morphophysiological parameters (length and number of rootlets, fresh biomass of rootlets) in the genotypes of the 1st group after AA of seeds was 3.0-38.0%; 2.0-12.0% and 12.0-50.0%, respectively, as compared to the control. These seeds had higher SP. Seeds of the genotypes of the 2nd group showed the decrease of these parameters after AA-test of seeds by 29.0-35.0%; 5.0-7.0% and 14.0-39.0%, respectively, as compared to the control. The values of these parameters in the genotypes of the 3rd group decreased by 31.0-42.0%; 4.0-8.0% and 8.068.0%, respectively, as compared to the control. Electrical conductivity of test solutions with the aged seeds of different genotypes increased at the beginning of the experiment by 7.0-26.0% at the average as compared to the control, and after the severe thermal stress, this increase was 7.0-25.0% as compared to the control. These results comply with the literature data on the increase of electrical conductivity of seeds after AA of seeds. This parameter is often used as a test for assessment of SP of seeds. By the content of peroxidase enzyme in the seedling rootlets after AA of seeds, the significant increase of this parameter was observed as compared to the control that is characteristic under the influence of thermal stress. The enzyme content in test variants was higher as compared to the control by 44.0% at the average, depending on the genotype. With the help of AA-test of seeds, storage potential of collection accessions of Triticum aestivum L. was evaluated that is an important characteristic of seed material under the ex situ conservation.