Introduction Mobilization, introduction, conservation and maintenance of plant diversity ex situ conditions, is the principal direction in the work of the Laboratory Floriculture of Botanical Garden (Institute) of the ASM. Over the course of sixty years was formats unique collections, which include representatives of various fl oristic regions of the world. Now the gene pool of ornamental plants Botanical Garden is about 1500 species, subspecies, varieties, relating to the 253 genera and 66 families. The work quotes the data on the taxonomic, ecologic and geographic composition of the perennial plants collections in the Botanical Garden (Institute) of ASM and importance for landscape. Material and methods The objects of study are species and cultivars of ornamental perennial plants at the experimental fi elds in Botanical Garden of ASM. Introductions sources are native fl ora also Index Seminum, expeditions, etc. Phytogeographical analysis, the biological and ecological, taxonomic position were performed according to standard methods [1, 2, 3]. Results and discussions Mobilization and introduction of plants from different regions of the Earth is with more results, when the natural habitat of alohtone species is nearer to new living conditions [2, 4]. Adaptation mechanisms are very different and the results are not always positive. Therefore, the objectives of our research is the study of the biomorphological peculiarities in the new conditions of life, the development of techniques, methods of cultivation and multiplication, selecting of an assortment of ornamental plants resistant. Perennials are presented 860 species and cultivars of 50 families and 157 genera. The number of species and varieties are leading family Paeoniaceae Rudolphi - 250 taxa, Asteraceae Dum. - 150, Iridaceae Juss.- 130, Hemerocallidaceae - 63, Cannaceae Juss. - 30, Crassulaceae DC - 28, Lamiaceae Lindl. - 20. The most numerous genera collected in collections Iris, Paeonia, Hemerocallis, Eremurus, Chrysanthemum, Sedum, Canna. Analysis of the geographical origin of species refl ects the following picture: the species with the largest share are of the European fl ora - 35%, followed by Asian fl ora - 22% and of American fl ora - 24%. Species from Central and South Africa - 8%. Japan and China - 10%. Native fl ora of our country is represented in collections of about 70 species with ornamental value. About 35 species of perennial ornamental plants are rare or endangered. Conclusions Perennials are presented of 860 species and cultivars, of 50 families and 157 genera. Introduced successfully, the species and varieties are used not only for landscaping, but also selection work. Have been obtained over 200 indigenous varieties of Paeonia, Iris, Canna etc. The collection of ornamental perennials Botanical Garden is the largest in the our country and is the basis of research, and an example of the use of plants in landscaping. It is also an integral part of the education and training of young generation.