The Nepetoideae is the largest subfamily in Lamiaceae family, and comprise the majority of medicinal, aromatic and culinary species. The main sources of plant antioxidants belong to Nepetoideae (they contain rosmarinic acid and also have a high content in volatile terpenes).  Our studies aimed at the achievement of in vitro tissue culture multiplication of species from wild populations and experimental cultures as an integrated part what envisages the identification, characterization, conservation and sustainable capitalization of some varieties of Nepetoideae species with an optimum content in biologically active compounds, from Romania and R. of Moldova. For the in vitro multiplication protocols, Nepeta parviflora Bieb, and Agastache rugosa Kuntze species were envisaged. A. rugosa, alochthonous species, now also cultivated in Europe, due to its therapeutic importance, was recently studied for it bioactive compounds (mainly essential oil and rosmarinic acid), biological activity (anti-apoptotic, antioxidant, antifungal, antiviral), respectively for the capitalization through biotechnology of its biosynthetic potential [1,2].  For the Nepeta species, the micropropagation is also studied as a conservation method, multiplication and reintroduction of rare species, aspect which is of interest in programs for amelioration of local genetic resources in the wild flora. In vitro multiplication studies were developed for endemic threatened species – with medicinal value, for their reintroduction in natural habitats [3,4]. N. parviflora, is included in the Red List of higher plants from Romania as vulnerable (VU) and for R. Moldova is considered species threatened with extinction in the local flora (regional assessment for the Red Book of Republic of Moldova, 3rd edition is Vulnerable [VU]) known from several extant locations. The plant material (seeds and floriferous stems) used for the initiation of tissue cultures and phytochemical assessments was collected (Mai – June 2011-2013) from experimental cultures of Romania for A. rugosa and from wild populations in R. of Moldova for N. parviflora species. Phytochemical profile for the polyphenolic compounds and essential oil were performed by means of TLC, HPLC, GSM and Spectrophotometry. The initiation of calusogenesis, the multiplication of stem buds, the plant regeneration and rhysogenesis induction were achieved on different MS variants, supplemented with BAP (benzylaminopurine) and NAA (naphthaleneacetic acid), in different ratios and concentrations, depending on the stages of the in vitro culture. The acclimatization of the in vitro regenerated plants was achieved in hydroponic system, the plants being afterwards transferred in soil. The results on the multiplication through tissue cultures may be used on the establishment of the protocols for the in situ and ex situ multiplication for species with phytotherapeutic potential and economic importance (A. rugosa). For N. parviflora species the in vitro multiplication generates material for propagation which may be used in conservation studies through establishing ex situ collections and experimental variants (for morpho-physiological and phytochemical studies) and also for reintroduction strategies, in accordance with the principles of Plant introduction and sustainable use of plant resources.