The impact of zinc oxide nanoparticles on protein content and activity of some antioxidant enzymes at pigmented yeasts
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EFREMOVA, Nadejda; BESHLIU, Alina; USATYI, Agafia. The impact of zinc oxide nanoparticles on protein content and activity of some antioxidant enzymes at pigmented yeasts. In: Analele Universitatii din Oradea, Fascicula Biologie. 2019, nr. 1(26), pp. 34-37. ISSN 1224-5119.
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Analele Universitatii din Oradea, Fascicula Biologie
Numărul 1(26) / 2019 / ISSN 1224-5119

The impact of zinc oxide nanoparticles on protein content and activity of some antioxidant enzymes at pigmented yeasts


Pag. 34-37

Efremova Nadejda, Beshliu Alina, Usatyi Agafia
 
Institute of Microbiology and Biotechnology
 
Disponibil în IBN: 13 aprilie 2019


Rezumat

The oxidative stress presents alteration followed by impact of nanoparticles and contributes to imbalance between formation of reactive oxygen radicals and antioxidative defense mechanisms of cell. In this study were used two types of ZnO nanoparticles <50 nm and <100 nm. The influence of some ZnO nanoparticles on protein content and the activity of enzymes - catalase and superoxide dismutase at pigmented yeast strain has been studied. Regarding the study of ZnO nanoparticles (<50 nm) it was established the increase of protein content by 57% in comparison with control values up to concentration of 20 mg/L. The activity of superoxide dismutase and catalase measured in yeast biomass after exposure to ZnO nanoparticles was significantly increased in comparison with control values. Meanwhile, the most pronounced biological effect on protein metabolism and activity of antioxidant enzymes was established for ZnO nanoparticles with <50 nm particle size. The study has revealed that zinc oxide nanoparticles of smaller size (<50 nm) were more effective than those of larger size (<100 nm). Thus, it can be concluded that adaptive response mechanism of yeasts depends on ZnO nanoparticles size and concentration.

Cuvinte-cheie
Antioxidant enzymes, catalase, Protein content, Rhodotorula gracilis, superoxide dismutase, Yeasts cultivation, ZnO nanoparticles